1r2k

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(New page: 200px<br /><applet load="1r2k" size="450" color="white" frame="true" align="right" spinBox="true" caption="1r2k, resolution 2.1&Aring;" /> '''Crystal structure of ...)
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[[Image:1r2k.gif|left|200px]]<br /><applet load="1r2k" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1r2k, resolution 2.1&Aring;" />
 
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'''Crystal structure of MoaB from Escherichia coli'''<br />
 
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==Overview==
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==Crystal structure of MoaB from Escherichia coli==
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The moaABC operon of Escherichia coli is involved in early steps of the, biosynthesis of the molybdenum-binding cofactor molybdopterin, but the, precise functions of the cognate proteins are not known. The crystal, structure of the MoaB protein from E. coli was determined by multiple, anomalous dispersion at 2.1 angstroms A resolution and refined to an R, factor of 20.4% (Rfree = 25.0%). The protein is a 32-symmetric hexamer, with the monomers consisting of a central beta-sheet flanked by helices on, both sides. The overall fold of the monomer is similar to those of the, MogA protein of E. coli, the G-domains of rat and human gephyrin and the, G-domains of Cnx1 protein from A. thaliana, all of which are involved in, the insertion of an unknown molybdenum species into molybdopterin to form, the molybdenum cofactor. Furthermore, the MoaB protein shows significant, sequence similarity to the cinnamon protein from Drosophila melanogaster., In addition to other functions, all these proteins are involved in the, biosynthesis of the molybdenum cofactor and have been shown to bind, molybdopterin. The close structural homology to MogA and the gephyrin and, Cnx1 domains suggests that MoaB may bind a hitherto unidentified pterin, compound, possibly an intermediate in molybdopterin biosynthesis.
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<StructureSection load='1r2k' size='340' side='right'caption='[[1r2k]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1r2k]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R2K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1R2K FirstGlance]. <br>
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1R2K is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1R2K OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1r2k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r2k OCA], [https://pdbe.org/1r2k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1r2k RCSB], [https://www.ebi.ac.uk/pdbsum/1r2k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1r2k ProSAT]</span></td></tr>
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Structure of the molybdenum-cofactor biosynthesis protein MoaB of Escherichia coli., Bader G, Gomez-Ortiz M, Haussmann C, Bacher A, Huber R, Fischer M, Acta Crystallogr D Biol Crystallogr. 2004 Jun;60(Pt 6):1068-75. Epub 2004, May 21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15159566 15159566]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MOAB_ECOLI MOAB_ECOLI] May be involved in the biosynthesis of molybdopterin. Can bind GTP and has low GTPase activity. Can bind MPT, but has no MPT adenylyl transferase activity.<ref>PMID:18154309</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r2/1r2k_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1r2k ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Bacher, A.]]
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[[Category: Bacher A]]
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[[Category: Bader, G.]]
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[[Category: Bader G]]
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[[Category: Fischer, M.]]
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[[Category: Fischer M]]
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[[Category: Gomez-Ortiz, M.]]
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[[Category: Gomez-Ortiz M]]
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[[Category: Haussmann, C.]]
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[[Category: Haussmann C]]
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[[Category: Huber, R.]]
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[[Category: Huber R]]
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[[Category: SO4]]
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[[Category: alpha-beta]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 01:13:25 2007''
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Current revision

Crystal structure of MoaB from Escherichia coli

PDB ID 1r2k

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