1r30

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="1r30" size="450" color="white" frame="true" align="right" spinBox="true" caption="1r30, resolution 3.40&Aring;" /> '''The Crystal Structur...)
Current revision (08:20, 14 February 2024) (edit) (undo)
 
(13 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1r30.gif|left|200px]]<br /><applet load="1r30" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1r30, resolution 3.40&Aring;" />
 
-
'''The Crystal Structure of Biotin Synthase, an S-Adenosylmethionine-Dependent Radical Enzyme'''<br />
 
-
==Overview==
+
==The Crystal Structure of Biotin Synthase, an S-Adenosylmethionine-Dependent Radical Enzyme==
-
The crystal structure of biotin synthase from Escherichia coli in complex, with S-adenosyl-L-methionine and dethiobiotin has been determined to 3.4, angstrom resolution. This structure addresses how "AdoMet radical" or, "radical SAM" enzymes use Fe4S4 clusters and S-adenosyl-L-methionine to, generate organic radicals. Biotin synthase catalyzes the radical-mediated, insertion of sulfur into dethiobiotin to form biotin. The structure places, the substrates between the Fe4S4 cluster, essential for radical, generation, and the Fe2S2 cluster, postulated to be the source of sulfur, with both clusters in unprecedented coordination environments.
+
<StructureSection load='1r30' size='340' side='right'caption='[[1r30]], [[Resolution|resolution]] 3.40&Aring;' scene=''>
-
 
+
== Structural highlights ==
-
==About this Structure==
+
<table><tr><td colspan='2'>[[1r30]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R30 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1R30 FirstGlance]. <br>
-
1R30 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with SAM, SF4, FES, DTB and TRS as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Biotin_synthase Biotin synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.1.6 2.8.1.6] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1R30 OCA].
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.4&#8491;</td></tr>
-
 
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DTB:6-(5-METHYL-2-OXO-IMIDAZOLIDIN-4-YL)-HEXANOIC+ACID'>DTB</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
-
==Reference==
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1r30 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r30 OCA], [https://pdbe.org/1r30 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1r30 RCSB], [https://www.ebi.ac.uk/pdbsum/1r30 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1r30 ProSAT]</span></td></tr>
-
Crystal structure of biotin synthase, an S-adenosylmethionine-dependent radical enzyme., Berkovitch F, Nicolet Y, Wan JT, Jarrett JT, Drennan CL, Science. 2004 Jan 2;303(5654):76-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14704425 14704425]
+
</table>
-
[[Category: Biotin synthase]]
+
== Function ==
 +
[https://www.uniprot.org/uniprot/BIOB_ECOLI BIOB_ECOLI] Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.<ref>PMID:8142361</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r3/1r30_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1r30 ConSurf].
 +
<div style="clear:both"></div>
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Berkovitch, F.]]
+
[[Category: Berkovitch F]]
-
[[Category: Drennan, C.L.]]
+
[[Category: Drennan CL]]
-
[[Category: Jarrett, J.T.]]
+
[[Category: Jarrett JT]]
-
[[Category: Nicolet, Y.]]
+
[[Category: Nicolet Y]]
-
[[Category: Wan, J.T.]]
+
[[Category: Wan JT]]
-
[[Category: DTB]]
+
-
[[Category: FES]]
+
-
[[Category: SAM]]
+
-
[[Category: SF4]]
+
-
[[Category: TRS]]
+
-
[[Category: fes cluster]]
+
-
[[Category: sam radical protein]]
+
-
[[Category: tim barrel]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 01:13:54 2007''
+

Current revision

The Crystal Structure of Biotin Synthase, an S-Adenosylmethionine-Dependent Radical Enzyme

PDB ID 1r30

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1r30"
Personal tools