1r5b

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(New page: 200px<br /><applet load="1r5b" size="450" color="white" frame="true" align="right" spinBox="true" caption="1r5b, resolution 2.35&Aring;" /> '''Crystal structure an...)
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[[Image:1r5b.jpg|left|200px]]<br /><applet load="1r5b" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1r5b, resolution 2.35&Aring;" />
 
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'''Crystal structure analysis of sup35'''<br />
 
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==Overview==
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==Crystal structure analysis of sup35==
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Translation termination in eukaryotes is governed by two interacting, release factors, eRF1 and eRF3. The crystal structure of the, eEF1alpha-like region of eRF3 from S. pombe determined in three states, (free protein, GDP-, and GTP-bound forms) reveals an overall structure, that is similar to EF-Tu, although with quite different domain, arrangements. In contrast to EF-Tu, GDP/GTP binding to eRF3c does not, induce dramatic conformational changes, and Mg(2+) is not required for GDP, binding to eRF3c. Mg(2+) at higher concentration accelerates GDP release, suggesting a novel mechanism for nucleotide exchange on eRF3 from that of, other GTPases. Mapping sequence conservation onto the molecular surface, combined with mutagenesis analysis, identified the eRF1 binding region, and revealed an essential function for the C terminus of eRF3. The, N-terminal extension, rich in acidic amino acids, blocks the proposed eRF1, binding site, potentially regulating eRF1 binding to eRF3 in a competitive, manner.
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<StructureSection load='1r5b' size='340' side='right'caption='[[1r5b]], [[Resolution|resolution]] 2.35&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1r5b]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R5B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1R5B FirstGlance]. <br>
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1R5B is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1R5B OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.35&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1r5b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r5b OCA], [https://pdbe.org/1r5b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1r5b RCSB], [https://www.ebi.ac.uk/pdbsum/1r5b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1r5b ProSAT]</span></td></tr>
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==Reference==
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</table>
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Crystal structure and functional analysis of the eukaryotic class II release factor eRF3 from S. pombe., Kong C, Ito K, Walsh MA, Wada M, Liu Y, Kumar S, Barford D, Nakamura Y, Song H, Mol Cell. 2004 Apr 23;14(2):233-45. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15099522 15099522]
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== Function ==
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[https://www.uniprot.org/uniprot/ERF3_SCHPO ERF3_SCHPO] Involved in translation termination. Stimulates the activity of ERF1. Binds guanine nucleotides.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r5/1r5b_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1r5b ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Schizosaccharomyces pombe]]
[[Category: Schizosaccharomyces pombe]]
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[[Category: Single protein]]
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[[Category: Kong C]]
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[[Category: Kong, C.]]
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[[Category: Song H]]
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[[Category: Song, H.]]
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[[Category: gtpase]]
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[[Category: peptide release]]
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[[Category: translation termination]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 01:16:27 2007''
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Current revision

Crystal structure analysis of sup35

PDB ID 1r5b

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