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2w00
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 2w00 is ON HOLD until Paper Publication Authors: Lapkouski, M., Panjikar, S., Janscak, P., Ettrich, R., Kuta Smatanova, I., Csefalvay, E. Descripti...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of the HsdR subunit of the EcoR124I restriction enzyme in complex with ATP== | |
| + | <StructureSection load='2w00' size='340' side='right'caption='[[2w00]], [[Resolution|resolution]] 2.60Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[2w00]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W00 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2W00 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
| + | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Type_I_site-specific_deoxyribonuclease Type I site-specific deoxyribonuclease], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.3 3.1.21.3] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2w00 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w00 OCA], [https://pdbe.org/2w00 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2w00 RCSB], [https://www.ebi.ac.uk/pdbsum/2w00 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2w00 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w0/2w00_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2w00 ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Type I restriction-modification enzymes act as conventional adenine methylases on hemimethylated DNAs, but unmethylated recognition targets induce them to translocate thousands of base pairs before cleaving distant sites nonspecifically. The first crystal structure of a type I motor subunit responsible for translocation and cleavage suggests how the pentameric translocating complex is assembled and provides a structural framework for translocation of duplex DNA by RecA-like ATPase motors. | ||
| - | + | Structure of the motor subunit of type I restriction-modification complex EcoR124I.,Lapkouski M, Panjikar S, Janscak P, Smatanova IK, Carey J, Ettrich R, Csefalvay E Nat Struct Mol Biol. 2008 Dec 14. PMID:19079266<ref>PMID:19079266</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 2w00" style="background-color:#fffaf0;"></div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Bacillus coli migula 1895]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Type I site-specific deoxyribonuclease]] | ||
| + | [[Category: Csefalvay, E]] | ||
| + | [[Category: Ettrich, R]] | ||
| + | [[Category: Lapkouski, M]] | ||
| + | [[Category: Panjikar, S]] | ||
| + | [[Category: Smatanova, I Kuta]] | ||
| + | [[Category: Atp-binding]] | ||
| + | [[Category: Dna-binding]] | ||
| + | [[Category: Helicase]] | ||
| + | [[Category: Hydrolase]] | ||
| + | [[Category: Nucleotide-binding]] | ||
| + | [[Category: R ecor124i]] | ||
| + | [[Category: Restriction system]] | ||
| + | [[Category: Type i restriction-modification enzyme]] | ||
Current revision
Crystal structure of the HsdR subunit of the EcoR124I restriction enzyme in complex with ATP
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Categories: Bacillus coli migula 1895 | Large Structures | Type I site-specific deoxyribonuclease | Csefalvay, E | Ettrich, R | Lapkouski, M | Panjikar, S | Smatanova, I Kuta | Atp-binding | Dna-binding | Helicase | Hydrolase | Nucleotide-binding | R ecor124i | Restriction system | Type i restriction-modification enzyme
