1r5q

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(New page: 200px<br /><applet load="1r5q" size="450" color="white" frame="true" align="right" spinBox="true" caption="1r5q, resolution 2.0&Aring;" /> '''Crystal Structure Ana...)
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[[Image:1r5q.jpg|left|200px]]<br /><applet load="1r5q" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1r5q, resolution 2.0&Aring;" />
 
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'''Crystal Structure Analysis of Kai A from PCC7120'''<br />
 
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==Overview==
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==Crystal Structure Analysis of Kai A from PCC7120==
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The cyanobacterial clock proteins KaiA and KaiB are proposed as regulators, of the circadian rhythm in cyanobacteria. Mutations in both proteins have, been reported to alter or abolish circadian rhythmicity. Here, we present, molecular models of both KaiA and KaiB from the cyanobacteria Anabaena sp, PCC7120 deduced by crystal structure analysis, and we discuss how, clock-changing or abolishing mutations may cause their resulting circadian, phenotype. The overall fold of the KaiA monomer is that of a four-helix, bundle. KaiB, on the other hand, adopts an alpha-beta meander motif. Both, proteins purify and crystallize as dimers. While the folds of the two, proteins are clearly different, their size and some surface features of, the physiologically relevant dimers are very similar. Notably, the, functionally relevant residues Arg 69 of KaiA and Arg 23 of KaiB align, well in space. The apparent structural similarities suggest that KaiA and, KaiB may compete for a potential common binding site on KaiC.
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<StructureSection load='1r5q' size='340' side='right'caption='[[1r5q]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1r5q]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Nostoc_sp._PCC_7120_=_FACHB-418 Nostoc sp. PCC 7120 = FACHB-418]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R5Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1R5Q FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1r5q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r5q OCA], [https://pdbe.org/1r5q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1r5q RCSB], [https://www.ebi.ac.uk/pdbsum/1r5q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1r5q ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/KAIA_NOSS1 KAIA_NOSS1] Component of the KaiABC clock protein complex, which constitutes the main circadian regulator in cyanobacteria. The KaiABC complex may act as a promoter-nonspecific transcription regulator that represses transcription, possibly by acting on the state of chromosome compaction. In the complex, it enhances the phosphorylation status of KaiC. In contrast, the presence of KaiB in the complex decreases the phosphorylation status of KaiC, suggesting that KaiB acts by antagonizing the interaction between KaiA and KaiC. A KaiA dimer is sufficient to enhance KaiC hexamer phosphorylation (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r5/1r5q_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1r5q ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1R5Q is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Anabaena_sp. Anabaena sp.]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1R5Q OCA].
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*[[Circadian clock protein 3D structures|Circadian clock protein 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Anabaena circadian clock proteins KaiA and KaiB reveal a potential common binding site to their partner KaiC., Garces RG, Wu N, Gillon W, Pai EF, EMBO J. 2004 Apr 21;23(8):1688-98. Epub 2004 Apr 8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15071498 15071498]
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[[Category: Large Structures]]
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[[Category: Anabaena sp.]]
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[[Category: Nostoc sp. PCC 7120 = FACHB-418]]
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[[Category: Single protein]]
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[[Category: Garces RG]]
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[[Category: Garces, R.G.]]
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[[Category: Gillon W]]
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[[Category: Gillon, W.]]
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[[Category: Pai EF]]
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[[Category: Pai, E.F.]]
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[[Category: Wu N]]
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[[Category: Wu, N.]]
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[[Category: four-helix-bundle]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 01:17:02 2007''
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Current revision

Crystal Structure Analysis of Kai A from PCC7120

PDB ID 1r5q

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