2vjw

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{{Seed}}
 
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[[Image:2vjw.jpg|left|200px]]
 
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==crystal structure of the second GAF domain of DevS from Mycobacterium smegmatis==
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The line below this paragraph, containing "STRUCTURE_2vjw", creates the "Structure Box" on the page.
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<StructureSection load='2vjw' size='340' side='right'caption='[[2vjw]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2vjw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycolicibacterium_smegmatis Mycolicibacterium smegmatis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VJW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VJW FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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{{STRUCTURE_2vjw| PDB=2vjw | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vjw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vjw OCA], [https://pdbe.org/2vjw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vjw RCSB], [https://www.ebi.ac.uk/pdbsum/2vjw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vjw ProSAT]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vj/2vjw_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2vjw ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The DevS histidine kinase of Mycobacterium smegmatis contains tandem GAF domains (GAF-A and GAF-B) in its N-terminal sensory domain. The heme iron of DevS is in the ferrous state when purified and is resistant to autooxidation from a ferrous to a ferric state in the presence of O(2). The redox property of the heme and the results of sequence comparison analysis indicate that DevS of M. smegmatis is more closely related to DosT of Mycobacterium tuberculosis than DevS of M. tuberculosis. The binding of O(2) to the deoxyferrous heme led to a decrease in the autokinase activity of DevS, whereas NO binding did not. The regulation of DevS autokinase activity in response to O(2) and NO was not observed in the DevS derivatives lacking its heme, indicating that the ligand-binding state of the heme plays an important role in the regulation of DevS kinase activity. The redox state of the quinone/quinol pool of the respiratory electron transport chain appears not to be implicated in the regulation of DevS activity. Neither cyclic GMP (cGMP) nor cAMP affected DevS autokinase activity, excluding the possibility that the cyclic nucleotides serve as the effector molecules to modulate DevS kinase activity. The three-dimensional structure of the putative GAF-B domain revealed that it has a GAF folding structure without cyclic nucleotide binding capacity.
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===CRYSTAL STRUCTURE OF THE SECOND GAF DOMAIN OF DEVS FROM MYCOBACTERIUM SMEGMATIS===
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O2- and NO-sensing mechanism through the DevSR two-component system in Mycobacterium smegmatis.,Lee JM, Cho HY, Cho HJ, Ko IJ, Park SW, Baik HS, Oh JH, Eom CY, Kim YM, Kang BS, Oh JI J Bacteriol. 2008 Oct;190(20):6795-804. Epub 2008 Aug 15. PMID:18708494<ref>PMID:18708494</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==About this Structure==
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</div>
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2VJW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_smegmatis Mycobacterium smegmatis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VJW OCA].
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<div class="pdbe-citations 2vjw" style="background-color:#fffaf0;"></div>
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[[Category: Mycobacterium smegmatis]]
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== References ==
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[[Category: Single protein]]
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<references/>
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[[Category: Cho, H J.]]
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__TOC__
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[[Category: Cho, H Y.]]
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</StructureSection>
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[[Category: Kang, B S.]]
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[[Category: Large Structures]]
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[[Category: Gaf]]
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[[Category: Mycolicibacterium smegmatis]]
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[[Category: Histidine kinase]]
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[[Category: Cho HJ]]
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[[Category: Hydrolase]]
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[[Category: Cho HY]]
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[[Category: Hypoxia sensing]]
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[[Category: Kang BS]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Aug 27 11:10:23 2008''
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Current revision

crystal structure of the second GAF domain of DevS from Mycobacterium smegmatis

PDB ID 2vjw

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