1r95

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="1r95" size="450" color="white" frame="true" align="right" spinBox="true" caption="1r95, resolution 2.65&Aring;" /> '''Crystal Structure of...)
Current revision (08:21, 14 February 2024) (edit) (undo)
 
(14 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1r95.jpg|left|200px]]<br /><applet load="1r95" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1r95, resolution 2.65&Aring;" />
 
-
'''Crystal Structure of IscA (native)'''<br />
 
-
==Overview==
+
==Crystal Structure of IscA (native)==
-
IscA belongs to an ancient family of proteins responsible for iron-sulfur, cluster assembly in essential metabolic pathways preserved throughout, evolution. We report here the 2.3 A resolution crystal structure of, Escherichia coli IscA, a novel fold in which mixed beta-sheets form a, compact alpha-beta sandwich domain. In contrast to the highly mobile, secondary structural elements within the bacterial Fe-S scaffold protein, IscU, a protein which is thought to have a similar function, the great, majority of the amino acids that are conserved in IscA homologues are, located in elements that constitute a well-ordered fold. However, the, 10-residue C-terminal tail segment that contains two invariant cysteines, critical for the Fe-S-binding function of a cyanobacterial (Synechocystis, PCC) IscA homologue is not ordered in our structure. In addition, the, crystal packing reveals a helical assembly that is constructed from two, possible tetrameric oligomers of IscA.
+
<StructureSection load='1r95' size='340' side='right'caption='[[1r95]], [[Resolution|resolution]] 2.65&Aring;' scene=''>
-
 
+
== Structural highlights ==
-
==About this Structure==
+
<table><tr><td colspan='2'>[[1r95]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R95 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1R95 FirstGlance]. <br>
-
1R95 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1R95 OCA].
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.65&#8491;</td></tr>
-
 
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1r95 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r95 OCA], [https://pdbe.org/1r95 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1r95 RCSB], [https://www.ebi.ac.uk/pdbsum/1r95 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1r95 ProSAT]</span></td></tr>
-
==Reference==
+
</table>
-
Crystal structure of the ancient, Fe-S scaffold IscA reveals a novel protein fold., Bilder PW, Ding H, Newcomer ME, Biochemistry. 2004 Jan 13;43(1):133-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14705938 14705938]
+
== Function ==
 +
[https://www.uniprot.org/uniprot/ISCA_ECOLI ISCA_ECOLI] Is able to transfer iron-sulfur clusters to apo-ferredoxin. Multiple cycles of [2Fe2S] cluster formation and transfer are observed, suggesting that IscA acts catalytically. Recruits intracellular free iron so as to provide iron for the assembly of transient iron-sulfur cluster in IscU in the presence of IscS, L-cysteine and the thioredoxin reductase system TrxA/TrxB.[HAMAP-Rule:MF_01429]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r9/1r95_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1r95 ConSurf].
 +
<div style="clear:both"></div>
 +
__TOC__
 +
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Bilder, P.W.]]
+
[[Category: Bilder PW]]
-
[[Category: Ding, H.]]
+
[[Category: Ding H]]
-
[[Category: Newcomer, M.E.]]
+
[[Category: Newcomer ME]]
-
[[Category: beta barrel]]
+
-
[[Category: iron-sulfur cluster protein]]
+
-
[[Category: pseudo-asymmetric motifs]]
+
-
[[Category: tetrameric]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 01:21:49 2007''
+

Current revision

Crystal Structure of IscA (native)

PDB ID 1r95

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1r95"
Personal tools