2nny

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{{Seed}}
 
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[[Image:2nny.png|left|200px]]
 
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==Crystal structure of the Ets1 dimer DNA complex.==
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The line below this paragraph, containing "STRUCTURE_2nny", creates the "Structure Box" on the page.
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<StructureSection load='2nny' size='340' side='right'caption='[[2nny]], [[Resolution|resolution]] 2.58&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2nny]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NNY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NNY FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.58&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2nny FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nny OCA], [https://pdbe.org/2nny PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2nny RCSB], [https://www.ebi.ac.uk/pdbsum/2nny PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2nny ProSAT]</span></td></tr>
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{{STRUCTURE_2nny| PDB=2nny | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ETS1_HUMAN ETS1_HUMAN] Transcription factor.<ref>PMID:10698492</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nn/2nny_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2nny ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The function of the Ets-1 transcription factor is regulated by two regions that flank its DNA-binding domain. A previously established mechanism for auto-inhibition of monomeric Ets-1 on DNA response elements with a single ETS-binding site, however, has not been observed for the stromelysin-1 promoter containing two palindromic ETS-binding sites. We present the structure of Ets-1 on this promoter element, revealing a ternary complex in which protein homo-dimerization is mediated by the specific arrangement of the two ETS-binding sites. In this complex, the N-terminal-flanking region is required for ternary protein-DNA assembly. Ets-1 variants, in which residues from this region are mutated, loose the ability for DNA-mediated dimerization and stromelysin-1 promoter transactivation. Thus, our data unravel the molecular basis for relief of auto-inhibition and the ability of Ets-1 to function as a facultative dimeric transcription factor on this site. Our findings may also explain previous data of Ets-1 function in the context of heterologous transcription factors, thus providing a molecular model that could also be valid for Ets-1 regulation by hetero-oligomeric assembly.
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===Crystal structure of the Ets1 dimer DNA complex.===
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Regulation of the transcription factor Ets-1 by DNA-mediated homo-dimerization.,Lamber EP, Vanhille L, Textor LC, Kachalova GS, Sieweke MH, Wilmanns M EMBO J. 2008 Jul 23;27(14):2006-17. Epub 2008 Jun 19. PMID:18566588<ref>PMID:18566588</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2nny" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_18566588}}, adds the Publication Abstract to the page
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*[[Ets1|Ets1]]
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(as it appears on PubMed at http://www.pubmed.gov), where 18566588 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_18566588}}
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__TOC__
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</StructureSection>
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==About this Structure==
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2NNY is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NNY OCA].
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==Reference==
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Regulation of the transcription factor Ets-1 by DNA-mediated homo-dimerization., Lamber EP, Vanhille L, Textor LC, Kachalova GS, Sieweke MH, Wilmanns M, EMBO J. 2008 Jul 23;27(14):2006-17. Epub 2008 Jun 19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18566588 18566588]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Kachalova, G S.]]
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[[Category: Kachalova GS]]
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[[Category: Lamber, E P.]]
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[[Category: Lamber EP]]
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[[Category: Wilmanns, M.]]
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[[Category: Wilmanns M]]
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[[Category: Ets-1]]
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[[Category: Protein-dna complex]]
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[[Category: Transcription/dna complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Sep 10 09:08:34 2008''
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Current revision

Crystal structure of the Ets1 dimer DNA complex.

PDB ID 2nny

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OCA

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