1rc7

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(New page: 200px<br /><applet load="1rc7" size="450" color="white" frame="true" align="right" spinBox="true" caption="1rc7, resolution 2.15&Aring;" /> '''Crystal structure of...)
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[[Image:1rc7.gif|left|200px]]<br /><applet load="1rc7" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1rc7, resolution 2.15&Aring;" />
 
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'''Crystal structure of RNase III Mutant E110K from Aquifex Aeolicus complexed with ds-RNA at 2.15 Angstrom Resolution'''<br />
 
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==Overview==
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==Crystal structure of RNase III Mutant E110K from Aquifex Aeolicus complexed with ds-RNA at 2.15 Angstrom Resolution==
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Ribonuclease III (RNase III) represents a family of double-stranded RNA, (dsRNA) endonucleases. The simplest bacterial enzyme contains an, endonuclease domain (endoND) and a dsRNA binding domain (dsRBD). RNase III, can affect RNA structure and gene expression in either of two ways: as a, dsRNA-processing enzyme that cleaves dsRNA, or as a dsRNA binding protein, that binds but does not cleave dsRNA. We previously determined the endoND, structure of Aquifex aeolicus RNase III (Aa-RNase III) and modeled a, catalytic complex of full-length Aa-RNase III with dsRNA. Here, we present, the crystal structure of Aa-RNase III in complex with dsRNA, revealing a, noncatalytic assembly. The major differences between the two functional, forms of RNase III.dsRNA are the conformation of the protein and the, orientation and location of dsRNA. The flexibility of a 7 residue linker, between the endoND and dsRBD enables the transition between these two, forms.
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<StructureSection load='1rc7' size='340' side='right'caption='[[1rc7]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1rc7]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RC7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RC7 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rc7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rc7 OCA], [https://pdbe.org/1rc7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rc7 RCSB], [https://www.ebi.ac.uk/pdbsum/1rc7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rc7 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RNC_AQUAE RNC_AQUAE] Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Also processes some mRNAs, and tRNAs when they are encoded in the rRNA operon.<ref>PMID:21138964</ref> <ref>PMID:16439209</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rc/1rc7_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rc7 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Ribonuclease III (RNase III) represents a family of double-stranded RNA (dsRNA) endonucleases. The simplest bacterial enzyme contains an endonuclease domain (endoND) and a dsRNA binding domain (dsRBD). RNase III can affect RNA structure and gene expression in either of two ways: as a dsRNA-processing enzyme that cleaves dsRNA, or as a dsRNA binding protein that binds but does not cleave dsRNA. We previously determined the endoND structure of Aquifex aeolicus RNase III (Aa-RNase III) and modeled a catalytic complex of full-length Aa-RNase III with dsRNA. Here, we present the crystal structure of Aa-RNase III in complex with dsRNA, revealing a noncatalytic assembly. The major differences between the two functional forms of RNase III.dsRNA are the conformation of the protein and the orientation and location of dsRNA. The flexibility of a 7 residue linker between the endoND and dsRBD enables the transition between these two forms.
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==About this Structure==
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Noncatalytic assembly of ribonuclease III with double-stranded RNA.,Blaszczyk J, Gan J, Tropea JE, Court DL, Waugh DS, Ji X Structure. 2004 Mar;12(3):457-66. PMID:15016361<ref>PMID:15016361</ref>
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1RC7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus] with TRS as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Ribonuclease_III Ribonuclease III], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.26.3 3.1.26.3] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1RC7 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Noncatalytic assembly of ribonuclease III with double-stranded RNA., Blaszczyk J, Gan J, Tropea JE, Court DL, Waugh DS, Ji X, Structure. 2004 Mar;12(3):457-66. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15016361 15016361]
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</div>
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[[Category: Aquifex aeolicus]]
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<div class="pdbe-citations 1rc7" style="background-color:#fffaf0;"></div>
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[[Category: Ribonuclease III]]
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[[Category: Single protein]]
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[[Category: Blaszczyk, J.]]
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[[Category: Gan, J.]]
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[[Category: Ji, X.]]
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[[Category: TRS]]
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[[Category: ds-rna]]
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[[Category: endonucleolytic cleavage]]
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[[Category: ribonuclease iii]]
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[[Category: rna interference]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 01:27:20 2007''
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==See Also==
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*[[Ribonuclease 3D structures|Ribonuclease 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Aquifex aeolicus]]
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[[Category: Large Structures]]
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[[Category: Blaszczyk J]]
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[[Category: Gan J]]
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[[Category: Ji X]]

Current revision

Crystal structure of RNase III Mutant E110K from Aquifex Aeolicus complexed with ds-RNA at 2.15 Angstrom Resolution

PDB ID 1rc7

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