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1rcf

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(New page: 200px<br /><applet load="1rcf" size="450" color="white" frame="true" align="right" spinBox="true" caption="1rcf, resolution 1.40&Aring;" /> '''STRUCTURE OF THE TRI...)
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[[Image:1rcf.jpg|left|200px]]<br /><applet load="1rcf" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1rcf, resolution 1.40&Aring;" />
 
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'''STRUCTURE OF THE TRIGONAL FORM OF RECOMBINANT OXIDIZED FLAVODOXIN FROM ANABAENA 7120 AT 1.40 ANGSTROMS RESOLUTION'''<br />
 
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==Overview==
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==STRUCTURE OF THE TRIGONAL FORM OF RECOMBINANT OXIDIZED FLAVODOXIN FROM ANABAENA 7120 AT 1.40 ANGSTROMS RESOLUTION==
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The oxidized recombinant flavodoxin from the cyanobacterium Anabaena 7120, has been crystallized in a trigonal form. The recombinant protein has an, identical primary structure to that purified directly from Anabaena, which, functions as a substitute for ferredoxin in an iron-deficient environment, for electron transfer from photosystem I to ferredoxin-NADP(+) reductase., X-ray data to 1.40 A were collected on a Siemens area detector. Of the 311, 379 reflections collected, 36069 reflections were unique in space group, P3(1)21 (a = 55.36, c = 102.59 A) with an R(merge) of 3.8%. The structure, was solved by molecular replacement using coordinates from the wild-type, monoclinic structure previously solved in this laboratory [Rao, Shaffie, Yu, Satyshur, Stockman &amp; Markley (1992). Protein Sci. 1, 1413-1427]. The, structure was refined with X-PLOR and SHELXL93 to a crystallographic, R-factor of 13.9% for 32963 reflections with I&gt; 2sigma(I). The final, structure contains 2767 atoms including 31 flavin mononucleotide (FMN), atoms, 299 water molecules, and one sulfate ion. The protein is comprised, of a central five-stranded beta-sheet surrounded by five helices and binds, a single molecule of FMN at the C-terminus of the sheet. The trigonal, protein structure and the crystal packing are compared with the monoclinic, wild-type protein. Helix alpha3 in this structure is less distorted than, in the monoclinic structure and shows additional hydrogen bonds in the, N-terminal portion of the helix. The trigonal structure is extensively, hydrogen bonded in three major areas with neighboring molecules compared, with five regions in the monoclinic structure, but using significantly, fewer hydrogen bonds to stabilize the lattice. There are several hydrogen, bonds to the amide groups from water molecules several of which stabilize, and extend the ends of the beta-sheet.
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<StructureSection load='1rcf' size='340' side='right'caption='[[1rcf]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1rcf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Nostoc_sp._PCC_7120_=_FACHB-418 Nostoc sp. PCC 7120 = FACHB-418]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RCF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RCF FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rcf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rcf OCA], [https://pdbe.org/1rcf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rcf RCSB], [https://www.ebi.ac.uk/pdbsum/1rcf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rcf ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/FLAV_NOSS1 FLAV_NOSS1] Low-potential electron donor to a number of redox enzymes.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rc/1rcf_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rcf ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1RCF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Anabaena_sp. Anabaena sp.] with SO4 and FMN as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1RCF OCA].
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*[[Flavodoxin 3D structures|Flavodoxin 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Structure of the trigonal form of recombinant oxidized flavodoxin from Anabaena 7120 at 1.40 A resolution., Burkhart BM, Ramakrishnan B, Yan H, Reedstrom RJ, Markley JL, Straus NA, Sundaralingam M, Acta Crystallogr D Biol Crystallogr. 1995 May 1;51(Pt 3):318-30. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15299298 15299298]
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[[Category: Large Structures]]
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[[Category: Anabaena sp.]]
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[[Category: Nostoc sp. PCC 7120 = FACHB-418]]
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[[Category: Single protein]]
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[[Category: Burkhart B]]
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[[Category: Burkhart, B.]]
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[[Category: Markley J]]
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[[Category: Markley, J.]]
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[[Category: Ramakrishnan B]]
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[[Category: Ramakrishnan, B.]]
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[[Category: Reedstrom R]]
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[[Category: Reedstrom, R.]]
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[[Category: Straus N]]
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[[Category: Straus, N.]]
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[[Category: Sundaralingam M]]
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[[Category: Sundaralingam, M.]]
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[[Category: Yan H]]
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[[Category: Yan, H.]]
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[[Category: FMN]]
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[[Category: SO4]]
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[[Category: electron transfer(flavoprotein)]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 01:27:47 2007''
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Current revision

STRUCTURE OF THE TRIGONAL FORM OF RECOMBINANT OXIDIZED FLAVODOXIN FROM ANABAENA 7120 AT 1.40 ANGSTROMS RESOLUTION

PDB ID 1rcf

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