1rf6

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(New page: 200px<br /><applet load="1rf6" size="450" color="white" frame="true" align="right" spinBox="true" caption="1rf6, resolution 1.9&Aring;" /> '''Structural Studies of...)
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[[Image:1rf6.jpg|left|200px]]<br /><applet load="1rf6" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1rf6, resolution 1.9&Aring;" />
 
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'''Structural Studies of Streptococcus pneumoniae EPSP Synthase in S3P-GLP Bound State'''<br />
 
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==Overview==
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==Structural Studies of Streptococcus pneumoniae EPSP Synthase in S3P-GLP Bound State==
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The shikimate pathway synthesizes aromatic amino acids and other essential, metabolites that are necessary for bacteria, plants and fungi to survive., This pathway is not present in vertebrates and therefore represents an, attractive target for antibacterial agents. We have successfully, crystallized and solved the structure of unliganded, inhibitor-liganded, and tetrahedral intermediate (TI)-liganded forms of Streptococcus, pneumoniae EPSP synthase. The overall topology of the S. pneumoniae EPSP, synthase is similar to that of the Escherichia coli EPSP synthase. In, addition, the majority of residues responsible for ligand binding were, conserved between the two proteins. TI-liganded structure provides, absolute configuration of the C-2 atom from the F-PEP moiety of the, enzyme-bound intermediate and also defines key residues responsible for, the enzyme reaction. Comparison of the unliganded state and, substrate-bound state of the enzyme provides insights into the structural, mechanisms involved in dynamic events of ligand binding, domain movement, and closure. This structural study of the pathogenic bacteria S., pneumoniae EPSP synthase with inhibitor and TI will provide invaluable, information for the design of new-generation antibiotics.
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<StructureSection load='1rf6' size='340' side='right'caption='[[1rf6]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1rf6]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RF6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RF6 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GPJ:GLYPHOSATE'>GPJ</scene>, <scene name='pdbligand=S3P:SHIKIMATE-3-PHOSPHATE'>S3P</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rf6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rf6 OCA], [https://pdbe.org/1rf6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rf6 RCSB], [https://www.ebi.ac.uk/pdbsum/1rf6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rf6 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/AROA_STRPN AROA_STRPN]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rf/1rf6_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rf6 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1RF6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae] with GPJ and S3P as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/3-phosphoshikimate_1-carboxyvinyltransferase 3-phosphoshikimate 1-carboxyvinyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.19 2.5.1.19] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1RF6 OCA].
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*[[EPSP synthase 3D structures|EPSP synthase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Structural studies of Streptococcus pneumoniae EPSP synthase in unliganded state, tetrahedral intermediate-bound state and S3P-GLP-bound state., Park H, Hilsenbeck JL, Kim HJ, Shuttleworth WA, Park YH, Evans JN, Kang C, Mol Microbiol. 2004 Feb;51(4):963-71. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14763973 14763973]
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[[Category: Large Structures]]
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[[Category: 3-phosphoshikimate 1-carboxyvinyltransferase]]
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[[Category: Single protein]]
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[[Category: Streptococcus pneumoniae]]
[[Category: Streptococcus pneumoniae]]
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[[Category: Evans, J.N.]]
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[[Category: Evans JN]]
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[[Category: Hilsenbeck, J.L.]]
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[[Category: Hilsenbeck JL]]
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[[Category: Kang, C.]]
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[[Category: Kang C]]
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[[Category: Kim, H.J.]]
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[[Category: Kim HJ]]
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[[Category: Park, H.]]
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[[Category: Park H]]
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[[Category: Park, Y.H.]]
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[[Category: Park YH]]
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[[Category: Shuttleworth, W.A.]]
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[[Category: Shuttleworth WA]]
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[[Category: GPJ]]
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[[Category: S3P]]
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[[Category: epsp synthase]]
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[[Category: glyphosate]]
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[[Category: pep]]
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[[Category: s. pneumoniae]]
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[[Category: s3p]]
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[[Category: shikimate pathway]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 01:32:48 2007''
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Structural Studies of Streptococcus pneumoniae EPSP Synthase in S3P-GLP Bound State

PDB ID 1rf6

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