1rfb

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(New page: 200px<br /><applet load="1rfb" size="450" color="white" frame="true" align="right" spinBox="true" caption="1rfb, resolution 3.0&Aring;" /> '''CRYSTAL STRUCTURE OF ...)
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[[Image:1rfb.gif|left|200px]]<br /><applet load="1rfb" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1rfb, resolution 3.0&Aring;" />
 
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'''CRYSTAL STRUCTURE OF RECOMBINANT BOVINE INTERFERON-GAMMA AT 3.0 ANGSTROMS RESOLUTION'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF RECOMBINANT BOVINE INTERFERON-GAMMA AT 3.0 ANGSTROMS RESOLUTION==
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The three-dimensional crystal structure of recombinant bovine, interferon-gamma was determined using the multiple isomorphous replacement, method at 3.0 A and refined to an R factor of 19.2%. This protein, crystallizes in space group P2(1)2(1)2(1) with unit-cell parameters of a =, 42.8, b = 79.9 and c = 85.4 A. There is one functional dimer in the, asymmetric unit. The two polypeptide chains are related by a, non-crystallographic twofold symmetry axis. The secondary structure is, predominantly alpha-helical with extensive interdigitation of the, alpha-helical segments of the polypeptide chains that make up the dimer., The secondary structure, tertiary structure and topology of this molecule, are identical to the previously reported structures of recombinant rabbit, interferon-gamma and recombinant human interferon-gamma. The molecular, topology is also similar to that of murine interferon-beta. These, structural similarities strongly indicate the presence of a unique, topological feature (fold) among gamma-interferons from different species, and also among the different classes of interferons.
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<StructureSection load='1rfb' size='340' side='right'caption='[[1rfb]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1rfb]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. The August 2010 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Interferons'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2010_8 10.2210/rcsb_pdb/mom_2010_8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RFB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RFB FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rfb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rfb OCA], [https://pdbe.org/1rfb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rfb RCSB], [https://www.ebi.ac.uk/pdbsum/1rfb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rfb ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/IFNG_BOVIN IFNG_BOVIN] Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rf/1rfb_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rfb ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1RFB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1RFB OCA].
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*[[Interferon 3D structures|Interferon 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Structure of recombinant bovine interferon-gamma at 3.0 A resolution., Samudzi CT, Rubin JR, Acta Crystallogr D Biol Crystallogr. 1993 Nov 1;49(Pt 6):513-21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15299487 15299487]
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[[Category: Bos taurus]]
[[Category: Bos taurus]]
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[[Category: Single protein]]
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[[Category: Interferons]]
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[[Category: Rubin, J.R.]]
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[[Category: Large Structures]]
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[[Category: Samudzi, C.T.]]
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[[Category: RCSB PDB Molecule of the Month]]
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[[Category: glycoprotein]]
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[[Category: Rubin JR]]
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[[Category: Samudzi CT]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 01:33:04 2007''
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Current revision

CRYSTAL STRUCTURE OF RECOMBINANT BOVINE INTERFERON-GAMMA AT 3.0 ANGSTROMS RESOLUTION

PDB ID 1rfb

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