1rh5

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(New page: 200px<br /><applet load="1rh5" size="450" color="white" frame="true" align="right" spinBox="true" caption="1rh5, resolution 3.20&Aring;" /> '''The structure of a p...)
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[[Image:1rh5.gif|left|200px]]<br /><applet load="1rh5" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1rh5, resolution 3.20&Aring;" />
 
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'''The structure of a protein conducting channel'''<br />
 
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==Overview==
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==The structure of a protein conducting channel==
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A conserved heterotrimeric membrane protein complex, the Sec61 or SecY, complex, forms a protein-conducting channel, allowing polypeptides to be, transferred across or integrated into membranes. We report the crystal, structure of the complex from Methanococcus jannaschii at a resolution of, 3.2 A. The structure suggests that one copy of the heterotrimer serves as, a functional translocation channel. The alpha-subunit has two linked, halves, transmembrane segments 1-5 and 6-10, clamped together by the, gamma-subunit. A cytoplasmic funnel leading into the channel is plugged by, a short helix. Plug displacement can open the channel into an 'hourglass', with a ring of hydrophobic residues at its constriction. This ring may, form a seal around the translocating polypeptide, hindering the permeation, of other molecules. The structure also suggests mechanisms for, signal-sequence recognition and for the lateral exit of transmembrane, segments of nascent membrane proteins into lipid, and indicates binding, sites for partners that provide the driving force for translocation.
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<StructureSection load='1rh5' size='340' side='right'caption='[[1rh5]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1rh5]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RH5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RH5 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rh5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rh5 OCA], [https://pdbe.org/1rh5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rh5 RCSB], [https://www.ebi.ac.uk/pdbsum/1rh5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rh5 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SECY_METJA SECY_METJA] The central subunit of the protein translocation channel SecYEG. Consists of two halves formed by TMs 1-5 and 6-10. These two domains form a lateral gate at the front which open onto the bilayer between TMs 2 and 7, and are clamped together by SecE at the back. The channel is closed by both a pore ring composed of hydrophobic SecY resides and a short helix (helix 2A) on the extracellular side of the membrane which forms a plug. The plug probably moves laterally to allow the channel to open. The ring and the pore may move independently.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rh/1rh5_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rh5 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1RH5 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1RH5 OCA].
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*[[Preprotein translocase 3D structures|Preprotein translocase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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X-ray structure of a protein-conducting channel., Van den Berg B, Clemons WM Jr, Collinson I, Modis Y, Hartmann E, Harrison SC, Rapoport TA, Nature. 2004 Jan 1;427(6969):36-44. Epub 2003 Dec 3. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14661030 14661030]
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[[Category: Large Structures]]
[[Category: Methanocaldococcus jannaschii]]
[[Category: Methanocaldococcus jannaschii]]
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[[Category: Protein complex]]
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[[Category: Clemons Jr WM]]
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[[Category: Berg, B.van.den.]]
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[[Category: Collinson I]]
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[[Category: Collinson, I.]]
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[[Category: Harrison SC]]
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[[Category: Harrison, S.C.]]
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[[Category: Hartmann E]]
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[[Category: Hartmann, E.]]
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[[Category: Modis Y]]
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[[Category: Jr., W.M.Clemons.]]
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[[Category: Rapoport TA]]
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[[Category: Modis, Y.]]
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[[Category: Van den Berg B]]
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[[Category: Rapoport, T.A.]]
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[[Category: membrane protein]]
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[[Category: protein channels]]
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[[Category: protein translocation]]
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[[Category: secy]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 01:35:36 2007''
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Current revision

The structure of a protein conducting channel

PDB ID 1rh5

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