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1rkr

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(New page: 200px<br /><applet load="1rkr" size="450" color="white" frame="true" align="right" spinBox="true" caption="1rkr, resolution 2.45&Aring;" /> '''CRYSTAL STRUCTURE OF...)
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[[Image:1rkr.jpg|left|200px]]<br /><applet load="1rkr" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1rkr, resolution 2.45&Aring;" />
 
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'''CRYSTAL STRUCTURE OF AZURIN-I FROM ALCALIGENES XYLOSOXIDANS NCIMB 11015'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF AZURIN-I FROM ALCALIGENES XYLOSOXIDANS NCIMB 11015==
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Azurin I from Alcaligenes xylosoxidans NCIMB 11015 (AzN-I) was, crystallized by using PEG 4000 as a precipitant. The crystals belong to, the monoclinic crystal system and have a space group C2 with the unit-cell, parameters of a = 130.67, b = 54.26, c = 74.55 A, and beta = 95.99, degrees. The structure of AzN-I has been solved by the molecular, replacement method. Azurin II from the same bacterium (AzN-II) was chosen, as the initial structural model. The final crystallographic R value is, 17.3% and free R value is 23.6% for 10958 reflections at a resolution of, 2.45 A. The root-mean-square deviations for main-chain atoms range between, 0.19 and 0.26 A among the four independent molecules in the asymmetric, unit. The Cu atom is coordinated to Ndelta of His46 and His117 at 2.0 (1), and 1.9 (1) A, and to Sgamma of Cys112 at 2.2 (1) A, while the carbonyl O, atom of Gly45 and Sdelta of Met121 coordinate axially to Cu atom at 2.5, (1) and 3.1 (1) A, respectively. The Cu-N and Cu-S distances of AzN-I are, quite similar to those of AzN-II, however, the Cu-SO (Gly45) bond length, in AzN-I is 0.25 A shorter than the counterpart in AzN-II. The results, have been used to discuss the differences in the spectra of these two, proteins.
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<StructureSection load='1rkr' size='340' side='right'caption='[[1rkr]], [[Resolution|resolution]] 2.45&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1rkr]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Achromobacter_xylosoxidans Achromobacter xylosoxidans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RKR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RKR FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.45&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rkr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rkr OCA], [https://pdbe.org/1rkr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rkr RCSB], [https://www.ebi.ac.uk/pdbsum/1rkr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rkr ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/AZUR1_ALCXX AZUR1_ALCXX] Transfers electrons from cytochrome c551 to cytochrome oxidase.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rk/1rkr_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rkr ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1RKR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Achromobacter_xylosoxidans Achromobacter xylosoxidans] with CU as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1RKR OCA].
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*[[Azurin 3D structures|Azurin 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Structure of azurin I from the denitrifying bacterium Alcaligenes xylosoxidans NCIMB 11015 at 2.45 A resolution., Li C, Inoue T, Gotowda M, Suzuki S, Yamaguchi K, Kunishige K, Kai Y, Acta Crystallogr D Biol Crystallogr. 1998 May 1;54(Pt 3):347-54. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9761902 9761902]
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[[Category: Achromobacter xylosoxidans]]
[[Category: Achromobacter xylosoxidans]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Gotowda, M.]]
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[[Category: Gotowda M]]
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[[Category: Inoue, T.]]
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[[Category: Inoue T]]
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[[Category: Kai, Y.]]
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[[Category: Kai Y]]
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[[Category: Kataoka, K.]]
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[[Category: Kataoka K]]
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[[Category: Li, C.]]
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[[Category: Li C]]
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[[Category: Suzuki, S.]]
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[[Category: Suzuki S]]
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[[Category: Yamaguchi, K.]]
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[[Category: Yamaguchi K]]
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[[Category: CU]]
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[[Category: electron transport]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 01:40:23 2007''
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Current revision

CRYSTAL STRUCTURE OF AZURIN-I FROM ALCALIGENES XYLOSOXIDANS NCIMB 11015

PDB ID 1rkr

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