1rm0

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="1rm0" size="450" color="white" frame="true" align="right" spinBox="true" caption="1rm0, resolution 2.05&Aring;" /> '''Crystal Structure of...)
Current revision (08:24, 14 February 2024) (edit) (undo)
 
(14 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1rm0.gif|left|200px]]<br /><applet load="1rm0" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1rm0, resolution 2.05&Aring;" />
 
-
'''Crystal Structure of Myo-Inositol 1-Phosphate Synthase From Saccharomyces cerevisiae In Complex With NAD+ and 2-deoxy-D-glucitol 6-(E)-vinylhomophosphonate'''<br />
 
-
==Overview==
+
==Crystal Structure of Myo-Inositol 1-Phosphate Synthase From Saccharomyces cerevisiae In Complex With NAD+ and 2-deoxy-D-glucitol 6-(E)-vinylhomophosphonate==
-
1l-myo-inositol 1-phosphate (MIP) synthase catalyzes the conversion of, d-glucose 6-phosphate to 1l-myo-inositol 1-phosphate, the first and, rate-limiting step in the biosynthesis of all inositol-containing, compounds. It involves an oxidation, enolization, intramolecular aldol, cyclization, and reduction. Here we present the structure of MIP synthase, in complex with NAD(+) and a high-affinity inhibitor, 2-deoxy-d-glucitol, 6-(E)-vinylhomophosphonate. This structure reveals interactions between, the enzyme active site residues and the inhibitor that are significantly, different from that proposed for 2-deoxy-d-glucitol 6-phosphate in the, previously published structure of MIP synthase-NAD(+)-2-deoxy-d-glucitol, 6-phosphate. There are several other conformational changes in NAD(+) and, the enzyme active site as well. Based on the new structural data, we, propose a new and completely different mechanism for MIP synthase.
+
<StructureSection load='1rm0' size='340' side='right'caption='[[1rm0]], [[Resolution|resolution]] 2.05&Aring;' scene=''>
-
 
+
== Structural highlights ==
-
==About this Structure==
+
<table><tr><td colspan='2'>[[1rm0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RM0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RM0 FirstGlance]. <br>
-
1RM0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with MN, D6P and NAI as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Inositol-3-phosphate_synthase Inositol-3-phosphate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.5.1.4 5.5.1.4] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1RM0 OCA].
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.05&#8491;</td></tr>
-
 
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=D6P:(3,4,5,7-TETRAHYDROXY-HEPT-1-ENYL)-PHOSPHONIC+ACID'>D6P</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NAI:1,4-DIHYDRONICOTINAMIDE+ADENINE+DINUCLEOTIDE'>NAI</scene></td></tr>
-
==Reference==
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rm0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rm0 OCA], [https://pdbe.org/1rm0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rm0 RCSB], [https://www.ebi.ac.uk/pdbsum/1rm0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rm0 ProSAT]</span></td></tr>
-
The structure of the 1L-myo-inositol-1-phosphate synthase-NAD+-2-deoxy-D-glucitol 6-(E)-vinylhomophosphonate complex demands a revision of the enzyme mechanism., Jin X, Foley KM, Geiger JH, J Biol Chem. 2004 Apr 2;279(14):13889-95. Epub 2003 Dec 18. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14684747 14684747]
+
</table>
-
[[Category: Inositol-3-phosphate synthase]]
+
== Function ==
 +
[https://www.uniprot.org/uniprot/INO1_YEAST INO1_YEAST]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rm/1rm0_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rm0 ConSurf].
 +
<div style="clear:both"></div>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
-
[[Category: Single protein]]
+
[[Category: Foley KM]]
-
[[Category: Foley, K.M.]]
+
[[Category: Geiger JH]]
-
[[Category: Geiger, J.H.]]
+
[[Category: Jin X]]
-
[[Category: Jin, X.]]
+
-
[[Category: D6P]]
+
-
[[Category: MN]]
+
-
[[Category: NAI]]
+
-
[[Category: myo-inositol 1-phosphate synthase]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 01:42:09 2007''
+

Current revision

Crystal Structure of Myo-Inositol 1-Phosphate Synthase From Saccharomyces cerevisiae In Complex With NAD+ and 2-deoxy-D-glucitol 6-(E)-vinylhomophosphonate

PDB ID 1rm0

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1rm0"
Personal tools