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1rof

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(New page: 200px<br /><applet load="1rof" size="450" color="white" frame="true" align="right" spinBox="true" caption="1rof" /> '''NMR STUDY OF 4FE-4S FERREDOXIN OF THERMATOGA...)
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[[Image:1rof.gif|left|200px]]<br /><applet load="1rof" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1rof" />
 
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'''NMR STUDY OF 4FE-4S FERREDOXIN OF THERMATOGA MARITIMA'''<br />
 
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==Overview==
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==NMR STUDY OF 4FE-4S FERREDOXIN OF THERMATOGA MARITIMA==
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The solution structure of the 60-residue 1[Fe4-S4] ferredoxin from the, hyperthermophilic bacterium Thermotoga maritima was determined based on, 683 distance and 35 dihedral angle restraints that were obtained from NMR, data. In addition, data known from crystallographic studies of ferredoxins, was used for modeling of the iron-sulfur cluster and its environment. The, protein shows a globular fold very similar to the fold of the related, 1[Fe4-S4] ferredoxins from Desulfovibrio gigas and Desulfovibrio, africanus, and elements of regular secondary structure similar to those in, other ferredoxins were found in the T. maritima protein. In particular, the T. maritima protein displayed a beta-sheet structure made up of, strands located at the very NH(2) and COOH termini of the protein, and an, internal alpha-helix. The internal beta-sheet observed in the D. gigas and, D. africanus ferredoxins could not be confirmed in T. maritima ferredoxin, and is thus suggested to be only weakly present or even absent in this, protein. This result suggests that thermostability in ferredoxins is not, necessarily correlated with the content of stable elements of regular, secondary structure.
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<StructureSection load='1rof' size='340' side='right'caption='[[1rof]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1rof]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ROF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ROF FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rof FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rof OCA], [https://pdbe.org/1rof PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rof RCSB], [https://www.ebi.ac.uk/pdbsum/1rof PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rof ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/FER_THEMA FER_THEMA] Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ro/1rof_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rof ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1ROF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima] with SF4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ROF OCA].
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*[[Ferredoxin 3D structures|Ferredoxin 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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An NMR-derived model for the solution structure of oxidized Thermotoga maritima 1[Fe4-S4] ferredoxin., Sticht H, Wildegger G, Bentrop D, Darimont B, Sterner R, Rosch P, Eur J Biochem. 1996 May 1;237(3):726-35. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8647119 8647119]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Thermotoga maritima]]
[[Category: Thermotoga maritima]]
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[[Category: Bentrop, D.]]
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[[Category: Bentrop D]]
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[[Category: Darimont, B.]]
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[[Category: Darimont B]]
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[[Category: Roesch, P.]]
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[[Category: Roesch P]]
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[[Category: Sterner, R.]]
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[[Category: Sterner R]]
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[[Category: Sticht, H.]]
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[[Category: Sticht H]]
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[[Category: Wildegger, G.]]
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[[Category: Wildegger G]]
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[[Category: SF4]]
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[[Category: electron transport]]
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[[Category: iron-sulfur]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 01:45:10 2007''
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Current revision

NMR STUDY OF 4FE-4S FERREDOXIN OF THERMATOGA MARITIMA

PDB ID 1rof

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