1s1y

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(New page: 200px<br /><applet load="1s1y" size="450" color="white" frame="true" align="right" spinBox="true" caption="1s1y, resolution 1.6&Aring;" /> '''Photoactivated chromo...)
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[[Image:1s1y.jpg|left|200px]]<br /><applet load="1s1y" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1s1y, resolution 1.6&Aring;" />
 
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'''Photoactivated chromophore conformation in Photoactive Yellow Protein (E46Q mutant) from 10 microseconds to 3 milliseconds'''<br />
 
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==Overview==
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==Photoactivated chromophore conformation in Photoactive Yellow Protein (E46Q mutant) from 10 microseconds to 3 milliseconds==
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We use time-resolved crystallography to observe the structural progression, of a bacterial blue light photoreceptor throughout its photocycle. Data, were collected from 10 ns to 100 ms after photoactivation of the E46Q, mutant of photoactive yellow protein. Refinement of transient chromophore, conformations shows that the spectroscopically distinct intermediates are, formed via progressive disruption of the hydrogen bond network to the, chromophore. Although structural change occurs within a few nanoseconds on, and around the chromophore, it takes milliseconds for a distinct pattern, of tertiary structural change to fully progress through the entire, molecule, thus generating the putative signaling state. Remarkably, the, coupling between the chromophore conformation and the tertiary structure, of this small protein is not tight: there are leads and lags between, changes in the conformation of the chromophore and the protein tertiary, structure.
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<StructureSection load='1s1y' size='340' side='right'caption='[[1s1y]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1s1y]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Halorhodospira_halophila Halorhodospira halophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S1Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1S1Y FirstGlance]. <br>
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1S1Y is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Halorhodospira_halophila Halorhodospira halophila] with HC4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1S1Y OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HC4:4-HYDROXYCINNAMIC+ACID'>HC4</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1s1y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s1y OCA], [https://pdbe.org/1s1y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1s1y RCSB], [https://www.ebi.ac.uk/pdbsum/1s1y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1s1y ProSAT]</span></td></tr>
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Chromophore conformation and the evolution of tertiary structural changes in photoactive yellow protein., Anderson S, Srajer V, Pahl R, Rajagopal S, Schotte F, Anfinrud P, Wulff M, Moffat K, Structure. 2004 Jun;12(6):1039-45. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15274923 15274923]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PYP_HALHA PYP_HALHA] Photoactive blue light protein. Probably functions as a photoreceptor for a negative phototaxis response.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/s1/1s1y_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1s1y ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Halorhodospira halophila]]
[[Category: Halorhodospira halophila]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Anderson, S.]]
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[[Category: Anderson S]]
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[[Category: Anfinrud, P.]]
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[[Category: Anfinrud P]]
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[[Category: Moffat, K.]]
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[[Category: Moffat K]]
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[[Category: Pahl, R.]]
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[[Category: Pahl R]]
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[[Category: Rajagopal, S.]]
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[[Category: Rajagopal S]]
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[[Category: Schotte, F.]]
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[[Category: Schotte F]]
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[[Category: Srajer, V.]]
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[[Category: Srajer V]]
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[[Category: Wulff, M.]]
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[[Category: Wulff M]]
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[[Category: HC4]]
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[[Category: room temperature]]
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[[Category: time-resolved]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 02:02:38 2007''
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Current revision

Photoactivated chromophore conformation in Photoactive Yellow Protein (E46Q mutant) from 10 microseconds to 3 milliseconds

PDB ID 1s1y

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