1s68

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(New page: 200px<br /><applet load="1s68" size="450" color="white" frame="true" align="right" spinBox="true" caption="1s68, resolution 1.90&Aring;" /> '''Structure and Mechan...)
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[[Image:1s68.jpg|left|200px]]<br /><applet load="1s68" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1s68, resolution 1.90&Aring;" />
 
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'''Structure and Mechanism of RNA Ligase'''<br />
 
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==Overview==
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==Structure and Mechanism of RNA Ligase==
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T4 RNA ligase 2 (Rnl2) exemplifies an RNA ligase family that includes the, RNA editing ligases (RELs) of Trypanosoma and Leishmania. The Rnl2/REL, enzymes are defined by essential signature residues and a unique, C-terminal domain, which we show is essential for sealing of 3'-OH and, 5'-PO4 RNA ends by Rnl2, but not for ligase adenylation or phosphodiester, bond formation at a preadenylated AppRNA end. The N-terminal segment, Rnl2(1-249) of the 334 aa Rnl2 protein comprises an autonomous, adenylyltransferase/AppRNA ligase domain. We report the 1.9 A crystal, structure of the ligase domain with AMP bound at the active site, which, reveals a shared fold, catalytic mechanism, and evolutionary history for, RNA ligases, DNA ligases, and mRNA capping enzymes.
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<StructureSection load='1s68' size='340' side='right'caption='[[1s68]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1s68]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_T4 Escherichia virus T4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S68 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1S68 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1s68 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s68 OCA], [https://pdbe.org/1s68 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1s68 RCSB], [https://www.ebi.ac.uk/pdbsum/1s68 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1s68 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RLIG2_BPT4 RLIG2_BPT4] Catalyzes intramolecular and intermolecular RNA strand joining (in vitro). May play a role in the repair of nicked RNA molecules.<ref>PMID:12228725</ref> <ref>PMID:17018278</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/s6/1s68_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1s68 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1S68 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t4 Bacteriophage t4] with AMP as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1S68 OCA].
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*[[RNA ligase|RNA ligase]]
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== References ==
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==Reference==
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<references/>
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Structure and mechanism of RNA ligase., Ho CK, Wang LK, Lima CD, Shuman S, Structure. 2004 Feb;12(2):327-39. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14962393 14962393]
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__TOC__
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[[Category: Bacteriophage t4]]
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</StructureSection>
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[[Category: Single protein]]
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[[Category: Escherichia virus T4]]
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[[Category: Ho, C.K.]]
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[[Category: Large Structures]]
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[[Category: Lima, C.D.]]
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[[Category: Ho CK]]
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[[Category: Shuman, S.]]
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[[Category: Lima CD]]
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[[Category: Wang, L.K.]]
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[[Category: Shuman S]]
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[[Category: AMP]]
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[[Category: Wang LK]]
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[[Category: ribonucleic acid ligase]]
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[[Category: rna repair]]
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[[Category: t4]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 02:08:52 2007''
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Current revision

Structure and Mechanism of RNA Ligase

PDB ID 1s68

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