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1s7z

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(New page: 200px<br /><applet load="1s7z" size="450" color="white" frame="true" align="right" spinBox="true" caption="1s7z, resolution 1.83&Aring;" /> '''Structure of Ocr fro...)
Current revision (08:29, 1 May 2024) (edit) (undo)
 
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[[Image:1s7z.jpg|left|200px]]<br /><applet load="1s7z" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1s7z, resolution 1.83&Aring;" />
 
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'''Structure of Ocr from Bacteriophage T7'''<br />
 
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==Overview==
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==Structure of Ocr from Bacteriophage T7==
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We have solved, by X-ray crystallography to a resolution of 1.8 A, the, structure of a protein capable of mimicking approximately 20 base pairs of, B-form DNA. This ocr protein, encoded by gene 0.3 of bacteriophage T7, mimics the size and shape of a bent DNA molecule and the arrangement of, negative charges along the phosphate backbone of B-form DNA. We also, demonstrate that ocr is an efficient inhibitor in vivo of all known, families of the complex type I DNA restriction enzymes. Using atomic force, microscopy, we have also observed that type I enzymes induce a bend in DNA, of similar magnitude to the bend in the ocr molecule. This first structure, of an antirestriction protein demonstrates the construction of structural, mimetics of long segments of B-form DNA.
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<StructureSection load='1s7z' size='340' side='right'caption='[[1s7z]], [[Resolution|resolution]] 1.83&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1s7z]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_phage_T7 Escherichia phage T7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S7Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1S7Z FirstGlance]. <br>
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1S7Z is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t7 Bacteriophage t7] with CS as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1S7Z OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.83&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CS:CESIUM+ION'>CS</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1s7z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s7z OCA], [https://pdbe.org/1s7z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1s7z RCSB], [https://www.ebi.ac.uk/pdbsum/1s7z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1s7z ProSAT]</span></td></tr>
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Structure of Ocr from bacteriophage T7, a protein that mimics B-form DNA., Walkinshaw MD, Taylor P, Sturrock SS, Atanasiu C, Berge T, Henderson RM, Edwardson JM, Dryden DT, Mol Cell. 2002 Jan;9(1):187-94. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11804597 11804597]
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</table>
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[[Category: Bacteriophage t7]]
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== Function ==
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[[Category: Single protein]]
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[https://www.uniprot.org/uniprot/OCR_BPT7 OCR_BPT7]
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[[Category: Atanasiu, C.]]
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== Evolutionary Conservation ==
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[[Category: Berg, T.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Dryden, D.T.]]
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Check<jmol>
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[[Category: Edwardson, J.M.]]
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<jmolCheckbox>
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[[Category: Henderson, R.M.]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/s7/1s7z_consurf.spt"</scriptWhenChecked>
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[[Category: Sturrock, S.S.]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: Taylor, P.]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: Walkinshaw, M.D.]]
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</jmolCheckbox>
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[[Category: CS]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1s7z ConSurf].
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[[Category: all helical]]
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<div style="clear:both"></div>
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[[Category: gene regulation]]
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__TOC__
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</StructureSection>
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 02:11:16 2007''
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[[Category: Escherichia phage T7]]
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[[Category: Large Structures]]
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[[Category: Atanasiu C]]
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[[Category: Berg T]]
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[[Category: Dryden DT]]
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[[Category: Edwardson JM]]
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[[Category: Henderson RM]]
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[[Category: Sturrock SS]]
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[[Category: Taylor P]]
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[[Category: Walkinshaw MD]]

Current revision

Structure of Ocr from Bacteriophage T7

PDB ID 1s7z

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