1sa0

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(New page: 200px<br /><applet load="1sa0" size="450" color="white" frame="true" align="right" spinBox="true" caption="1sa0, resolution 3.58&Aring;" /> '''TUBULIN-COLCHICINE: ...)
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[[Image:1sa0.gif|left|200px]]<br /><applet load="1sa0" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1sa0, resolution 3.58&Aring;" />
 
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'''TUBULIN-COLCHICINE: STATHMIN-LIKE DOMAIN COMPLEX'''<br />
 
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==Overview==
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==TUBULIN-COLCHICINE: STATHMIN-LIKE DOMAIN COMPLEX==
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Microtubules are cytoskeletal polymers of tubulin involved in many, cellular functions. Their dynamic instability is controlled by numerous, compounds and proteins, including colchicine and stathmin family proteins., The way in which microtubule instability is regulated at the molecular, level has remained elusive, mainly because of the lack of appropriate, structural data. Here, we present the structure, at 3.5 A resolution, of, tubulin in complex with colchicine and with the stathmin-like domain (SLD), of RB3. It shows the interaction of RB3-SLD with two tubulin heterodimers, in a curved complex capped by the SLD amino-terminal domain, which, prevents the incorporation of the complexed tubulin into microtubules. A, comparison with the structure of tubulin in protofilaments shows changes, in the subunits of tubulin as it switches from its straight conformation, to a curved one. These changes correlate with the loss of lateral contacts, and provide a rationale for the rapid microtubule depolymerization, characteristic of dynamic instability. Moreover, the tubulin-colchicine, complex sheds light on the mechanism of colchicine's activity: we show, that colchicine binds at a location where it prevents curved tubulin from, adopting a straight structure, which inhibits assembly.
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<StructureSection load='1sa0' size='340' side='right'caption='[[1sa0]], [[Resolution|resolution]] 3.58&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1sa0]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SA0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SA0 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.58&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CN2:2-MERCAPTO-N-[1,2,3,10-TETRAMETHOXY-9-OXO-5,6,7,9-TETRAHYDRO-BENZO[A]HEPTALEN-7-YL]ACETAMIDE'>CN2</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sa0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sa0 OCA], [https://pdbe.org/1sa0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sa0 RCSB], [https://www.ebi.ac.uk/pdbsum/1sa0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sa0 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TBA1D_BOVIN TBA1D_BOVIN] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sa/1sa0_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sa0 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1SA0 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with MG, GTP, GDP and CN2 as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1SA0 OCA].
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*[[Stathmin-4 3D structures|Stathmin-4 3D structures]]
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*[[Tubulin 3D Structures|Tubulin 3D Structures]]
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==Reference==
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__TOC__
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Insight into tubulin regulation from a complex with colchicine and a stathmin-like domain., Ravelli RB, Gigant B, Curmi PA, Jourdain I, Lachkar S, Sobel A, Knossow M, Nature. 2004 Mar 11;428(6979):198-202. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15014504 15014504]
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</StructureSection>
[[Category: Bos taurus]]
[[Category: Bos taurus]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
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[[Category: Curmi, P.A.]]
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[[Category: Curmi PA]]
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[[Category: Gigant, B.]]
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[[Category: Gigant B]]
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[[Category: Jourdain, I.]]
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[[Category: Jourdain I]]
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[[Category: Knossow, M.]]
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[[Category: Knossow M]]
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[[Category: Lachkar, S.]]
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[[Category: Lachkar S]]
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[[Category: Ravelli, R.B.]]
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[[Category: Ravelli RB]]
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[[Category: Sobel, A.]]
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[[Category: Sobel A]]
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[[Category: CN2]]
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[[Category: GDP]]
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[[Category: GTP]]
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[[Category: MG]]
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[[Category: alpha-tubulin]]
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[[Category: beta-tubulin]]
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[[Category: colchicine]]
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[[Category: gtpase]]
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[[Category: microtubule podophyllotoxin]]
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[[Category: stathmin]]
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[[Category: tubulin]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 02:13:24 2007''
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Current revision

TUBULIN-COLCHICINE: STATHMIN-LIKE DOMAIN COMPLEX

PDB ID 1sa0

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