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1sbz

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(New page: 200px<br /><applet load="1sbz" size="450" color="white" frame="true" align="right" spinBox="true" caption="1sbz, resolution 2.00&Aring;" /> '''Crystal Structure of...)
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[[Image:1sbz.gif|left|200px]]<br /><applet load="1sbz" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1sbz, resolution 2.00&Aring;" />
 
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'''Crystal Structure of dodecameric FMN-dependent Ubix-like Decarboxylase from Escherichia coli O157:H7'''<br />
 
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==Overview==
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==Crystal Structure of dodecameric FMN-dependent Ubix-like Decarboxylase from Escherichia coli O157:H7==
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The crystal structure of the flavoprotein Pad1 from Escherichia coli, O157:H7 complexed with the cofactor FMN has been determined by the, multiple anomalous diffraction method and refined at 2.0 A resolution., This protein is a paralog of UbiX (3-octaprenyl-4-hydroxybenzoate, carboxylyase, 51% sequence identity) that catalyzes the third step in, ubiquinone biosynthesis and to Saccharomyces cerevisiae Pad1 (54%, identity), an enzyme that confers resistance to the antimicrobial, compounds phenylacrylic acids through decarboxylation of these compounds., Each Pad1 monomer consists of a typical Rossmann fold containing a, non-covalently bound molecule of FMN. The fold of Pad1 is similar to MrsD, an enzyme associated with lantibiotic synthesis; EpiD, a peptidyl-cysteine, decarboxylase; and AtHAL3a, the enzyme, which decarboxylates, 4'-phosphopantothenoylcysteine to 4'-phosphopantetheine during coenzyme A, biosynthesis, all with a similar location of the FMN binding site at the, interface between two monomers, yet each having little sequence similarity, to one another. All of these proteins associate into oligomers, with a, trimer forming the common structural unit in each case. In MrsD and EpiD, which belong to the homo-dodecameric flavin-containing cysteine, decarboxylase (HFCD) family, these trimers associate further into, dodecamers. Pad1 also forms dodecamers, although the association of the, trimers is completely different, resulting in exposure of a different side, of the trimer unit to the solvent. This exposure affects the location of, the substrate binding site and, specifically, its access to the FMN, cofactor. Therefore, Pad1 forms a separate family, distinguishable from, the HFCD family.
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<StructureSection load='1sbz' size='340' side='right'caption='[[1sbz]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1sbz]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_O157:H7 Escherichia coli O157:H7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SBZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SBZ FirstGlance]. <br>
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1SBZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with FMN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1SBZ OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sbz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sbz OCA], [https://pdbe.org/1sbz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sbz RCSB], [https://www.ebi.ac.uk/pdbsum/1sbz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sbz ProSAT], [https://www.topsan.org/Proteins/BSGI/1sbz TOPSAN]</span></td></tr>
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Crystal structure of a dodecameric FMN-dependent UbiX-like decarboxylase (Pad1) from Escherichia coli O157: H7., Rangarajan ES, Li Y, Iannuzzi P, Tocilj A, Hung LW, Matte A, Cygler M, Protein Sci. 2004 Nov;13(11):3006-16. Epub 2004 Sep 30. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15459342 15459342]
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</table>
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[[Category: Escherichia coli]]
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== Function ==
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[[Category: Single protein]]
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[https://www.uniprot.org/uniprot/PADL_ECO57 PADL_ECO57] Involved in the non-oxidative decarboxylation and detoxification of phenolic derivatives under both aerobic and anaerobic conditions (PubMed:15979273). Flavin prenyltransferase that catalyzes the synthesis of the prenylated FMN cofactor (prenyl-FMN) for phenolic acid decarboxylase (By similarity).[HAMAP-Rule:MF_01986]<ref>PMID:15979273</ref>
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[[Category: BSGI, Montreal-Kingston.Bacterial.Structural.Genomics.Initiative.]]
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== Evolutionary Conservation ==
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[[Category: Cygler, M.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Hung, L.W.]]
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Check<jmol>
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[[Category: Iannuzzi, P.]]
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<jmolCheckbox>
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[[Category: Li, Y.]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sb/1sbz_consurf.spt"</scriptWhenChecked>
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[[Category: Matte, A.]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: Rangarajan, E.S.]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: Tocilj, A.]]
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</jmolCheckbox>
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[[Category: FMN]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sbz ConSurf].
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[[Category: bsgi]]
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<div style="clear:both"></div>
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[[Category: fmn binding]]
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== References ==
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[[Category: montreal-kingston bacterial structural genomics initiative]]
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<references/>
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[[Category: pad1]]
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__TOC__
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[[Category: structural genomics]]
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</StructureSection>
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[[Category: ubix]]
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[[Category: Escherichia coli O157:H7]]
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[[Category: Large Structures]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 02:15:49 2007''
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[[Category: Cygler M]]
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[[Category: Hung L-W]]
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[[Category: Iannuzzi P]]
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[[Category: Li Y]]
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[[Category: Matte A]]
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[[Category: Rangarajan ES]]
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[[Category: Tocilj A]]

Current revision

Crystal Structure of dodecameric FMN-dependent Ubix-like Decarboxylase from Escherichia coli O157:H7

PDB ID 1sbz

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