1scb

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(New page: 200px<br /><applet load="1scb" size="450" color="white" frame="true" align="right" spinBox="true" caption="1scb, resolution 2.3&Aring;" /> '''ENZYME CRYSTAL STRUCT...)
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[[Image:1scb.gif|left|200px]]<br /><applet load="1scb" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1scb, resolution 2.3&Aring;" />
 
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'''ENZYME CRYSTAL STRUCTURE IN A NEAT ORGANIC SOLVENT'''<br />
 
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==Overview==
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==ENZYME CRYSTAL STRUCTURE IN A NEAT ORGANIC SOLVENT==
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The crystal structure of the serine protease subtilisin Carlsberg in, anhydrous acetonitrile was determined at 2.3 A resolution. It was found to, be essentially identical to the three-dimensional structure of the enzyme, in water; the differences observed were smaller than those between two, independently determined structures in aqueous solution. The hydrogen bond, system of the catalytic triad is intact in acetonitrile. The majority (99, of 119) of enzyme-bound, structural water molecules have such a great, affinity to subtilisin that they are not displaced even in anhydrous, acetonitrile. Of the 12 enzyme-bound acetonitrile molecules, 4 displace, water molecules and 8 bind where no water had been observed before., One-third of all subtilisin-bound acetonitrile molecules reside in the, active center, occupying the same region (P1, P2, and P3 binding sites) as, the specific protein inhibitor eglin c.
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<StructureSection load='1scb' size='340' side='right'caption='[[1scb]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1scb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_licheniformis Bacillus licheniformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SCB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SCB FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CCN:ACETONITRILE'>CCN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1scb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1scb OCA], [https://pdbe.org/1scb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1scb RCSB], [https://www.ebi.ac.uk/pdbsum/1scb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1scb ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SUBC_BACLI SUBC_BACLI] Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides (Ref.4, PubMed:11109488). Shows high specificity for aromatic and hydrophobic amino acids in the P1 substrate position (PubMed:11109488). May play an important role in the degradation of feather keratin (PubMed:11109488).<ref>PMID:11109488</ref> <ref>PMID:4967581</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sc/1scb_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1scb ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1SCB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_licheniformis Bacillus licheniformis] with CA and CCN as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Subtilisin Subtilisin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.62 3.4.21.62] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1SCB OCA].
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*[[Subtilisin 3D structures|Subtilisin 3D structures]]
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== References ==
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==Reference==
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<references/>
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Enzyme crystal structure in a neat organic solvent., Fitzpatrick PA, Steinmetz AC, Ringe D, Klibanov AM, Proc Natl Acad Sci U S A. 1993 Sep 15;90(18):8653-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8378343 8378343]
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__TOC__
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</StructureSection>
[[Category: Bacillus licheniformis]]
[[Category: Bacillus licheniformis]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Subtilisin]]
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[[Category: Fitzpatrick PA]]
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[[Category: Fitzpatrick, P.A.]]
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[[Category: Klibanov AM]]
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[[Category: Klibanov, A.M.]]
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[[Category: Ringe D]]
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[[Category: Ringe, D.]]
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[[Category: Steinmetz ACU]]
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[[Category: Steinmetz, A.C.U.]]
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[[Category: CA]]
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[[Category: CCN]]
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[[Category: serine protease]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 02:16:12 2007''
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ENZYME CRYSTAL STRUCTURE IN A NEAT ORGANIC SOLVENT

PDB ID 1scb

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