1sfc

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(New page: 200px<br /><applet load="1sfc" size="450" color="white" frame="true" align="right" spinBox="true" caption="1sfc, resolution 2.40&Aring;" /> '''NEURONAL SYNAPTIC FU...)
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[[Image:1sfc.gif|left|200px]]<br /><applet load="1sfc" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1sfc, resolution 2.40&Aring;" />
 
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'''NEURONAL SYNAPTIC FUSION COMPLEX'''<br />
 
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==Overview==
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==NEURONAL SYNAPTIC FUSION COMPLEX==
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The evolutionarily conserved SNARE proteins and their complexes are, involved in the fusion of vesicles with their target membranes; however, the overall organization and structural details of these complexes are, unknown. Here we report the X-ray crystal structure at 2.4 A resolution of, a core synaptic fusion complex containing syntaxin-1 A, synaptobrevin-II, and SNAP-25B. The structure reveals a highly twisted and parallel, four-helix bundle that differs from the bundles described for the, haemagglutinin and HIV/SIV gp41 membrane-fusion proteins. Conserved, leucine-zipper-like layers are found at the centre of the synaptic fusion, complex. Embedded within these leucine-zipper layers is an ionic layer, consisting of an arginine and three glutamine residues contributed from, each of the four alpha-helices. These residues are highly conserved across, the entire SNARE family. The regions flanking the leucine-zipper-like, layers contain a hydrophobic core similar to that of more general, four-helix-bundle proteins. The surface of the synaptic fusion complex is, highly grooved and possesses distinct hydrophilic, hydrophobic and charged, regions. These characteristics may be important for membrane fusion and, for the binding of regulatory factors affecting neurotransmission.
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<StructureSection load='1sfc' size='340' side='right'caption='[[1sfc]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1sfc]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. The November 2013 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''SNARE Proteins'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2013_11 10.2210/rcsb_pdb/mom_2013_11]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SFC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SFC FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=SR:STRONTIUM+ION'>SR</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sfc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sfc OCA], [https://pdbe.org/1sfc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sfc RCSB], [https://www.ebi.ac.uk/pdbsum/1sfc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sfc ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/VAMP2_RAT VAMP2_RAT] Involved in the targeting and/or fusion of transport vesicles to their target membrane (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sf/1sfc_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sfc ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1SFC is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with SR and MPD as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1SFC OCA].
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*[[Syntaxin 3D structures|Syntaxin 3D structures]]
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*[[Vesicle-associated membrane protein|Vesicle-associated membrane protein]]
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==Reference==
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__TOC__
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Crystal structure of a SNARE complex involved in synaptic exocytosis at 2.4 A resolution., Sutton RB, Fasshauer D, Jahn R, Brunger AT, Nature. 1998 Sep 24;395(6700):347-53. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9759724 9759724]
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</StructureSection>
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: RCSB PDB Molecule of the Month]]
[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
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[[Category: Brunger, A.T.]]
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[[Category: SNARE Proteins]]
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[[Category: Sutton, R.B.]]
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[[Category: Brunger AT]]
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[[Category: MPD]]
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[[Category: Sutton RB]]
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[[Category: SR]]
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[[Category: membrane fusion protein complex]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 02:20:34 2007''
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NEURONAL SYNAPTIC FUSION COMPLEX

PDB ID 1sfc

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