1sg3

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(New page: 200px<br /><applet load="1sg3" size="450" color="white" frame="true" align="right" spinBox="true" caption="1sg3, resolution 2.60&Aring;" /> '''Structure of allanto...)
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[[Image:1sg3.gif|left|200px]]<br /><applet load="1sg3" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1sg3, resolution 2.60&Aring;" />
 
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'''Structure of allantoicase'''<br />
 
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==Overview==
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==Structure of allantoicase==
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Allantoicase (EC 3.5.3.4) catalyzes the conversion of allantoate into, ureidoglycolate and urea, one of the final steps in the degradation of, purines to urea. The mechanism of most enzymes involved in this pathway, which has been known for a long time, is unknown. In this paper we, describe the three-dimensional crystal structure of the yeast allantoicase, determined at a resolution of 2.6 A by single anomalous diffraction. This, constitutes the first structure for an enzyme of this pathway. The, structure reveals a repeated jelly roll beta-sheet motif, also present in, proteins of unrelated biochemical function. Allantoicase has a hexameric, arrangement in the crystal (dimer of trimers). Analysis of the protein, sequence against the structural data reveals the presence of two totally, conserved surface patches, one on each jelly roll motif. The hexameric, packing concentrates these patches into conserved pockets that probably, constitute the active site.
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<StructureSection load='1sg3' size='340' side='right'caption='[[1sg3]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1sg3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SG3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SG3 FirstGlance]. <br>
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1SG3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Active as [http://en.wikipedia.org/wiki/Allantoicase Allantoicase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.3.4 3.5.3.4] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1SG3 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sg3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sg3 OCA], [https://pdbe.org/1sg3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sg3 RCSB], [https://www.ebi.ac.uk/pdbsum/1sg3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sg3 ProSAT]</span></td></tr>
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==Reference==
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</table>
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Crystal structure of yeast allantoicase reveals a repeated jelly roll motif., Leulliot N, Quevillon-Cheruel S, Sorel I, Graille M, Meyer P, Liger D, Blondeau K, Janin J, van Tilbeurgh H, J Biol Chem. 2004 May 28;279(22):23447-52. Epub 2004 Mar 12. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15020593 15020593]
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== Function ==
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[[Category: Allantoicase]]
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[https://www.uniprot.org/uniprot/ALLC_YEAST ALLC_YEAST] Utilization of purines as secondary nitrogen sources, when primary sources are limiting.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sg/1sg3_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sg3 ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Single protein]]
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[[Category: Blondeau K]]
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[[Category: Blondeau, K.]]
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[[Category: Graille M]]
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[[Category: Graille, M.]]
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[[Category: Janin J]]
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[[Category: Janin, J.]]
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[[Category: Leulliot N]]
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[[Category: Leulliot, N.]]
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[[Category: Liger D]]
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[[Category: Liger, D.]]
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[[Category: Meyer P]]
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[[Category: Meyer, P.]]
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[[Category: Quevillon-Cheruel S]]
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[[Category: Quevillon-Cheruel, S.]]
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[[Category: Sorel I]]
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[[Category: Sorel, I.]]
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[[Category: Van Tilbeurgh H]]
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[[Category: Tilbeurgh, H.van.]]
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[[Category: allantoicase]]
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[[Category: hexamer]]
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[[Category: jelly roll]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 02:21:40 2007''
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Current revision

Structure of allantoicase

PDB ID 1sg3

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