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1sig

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(New page: 200px<br /><applet load="1sig" size="450" color="white" frame="true" align="right" spinBox="true" caption="1sig, resolution 2.6&Aring;" /> '''CRYSTAL STRUCTURE OF ...)
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[[Image:1sig.jpg|left|200px]]<br /><applet load="1sig" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1sig, resolution 2.6&Aring;" />
 
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'''CRYSTAL STRUCTURE OF A SIGMA70 SUBUNIT FRAGMENT FROM ESCHERICHIA COLI RNA POLYMERASE'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF A SIGMA70 SUBUNIT FRAGMENT FROM ESCHERICHIA COLI RNA POLYMERASE==
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The 2.6 A crystal structure of a fragment of the sigma 70 promoter, specificity subunit of E. coli RNA polymerase is described. Residues, involved in core RNA polymerase binding lie on one face of the structure., On the opposite face, aligned along one helix, are exposed residues that, interact with the -10 consensus promoter element (the Pribnow box), including four aromatic residues involved in promoter melting. The, structure suggests one way in which DNA interactions may be inhibited in, the absence of RNA polymerase and provides a framework for the, interpretation of a large number of genetic and biochemical analyses.
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<StructureSection load='1sig' size='340' side='right'caption='[[1sig]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1sig]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SIG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SIG FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sig FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sig OCA], [https://pdbe.org/1sig PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sig RCSB], [https://www.ebi.ac.uk/pdbsum/1sig PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sig ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RPOD_ECOLI RPOD_ECOLI] Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/si/1sig_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sig ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1SIG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1SIG OCA].
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*[[Sigma factor 3D structures|Sigma factor 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure of a sigma 70 subunit fragment from E. coli RNA polymerase., Malhotra A, Severinova E, Darst SA, Cell. 1996 Oct 4;87(1):127-36. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8858155 8858155]
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[[Category: Escherichia coli K-12]]
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[[Category: Escherichia coli]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Darst SA]]
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[[Category: Darst, S.A.]]
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[[Category: Malhotra A]]
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[[Category: Malhotra, A.]]
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[[Category: Severinova E]]
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[[Category: Severinova, E.]]
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[[Category: rna polymerase sigma factor]]
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[[Category: transcription regulation]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 02:24:19 2007''
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Current revision

CRYSTAL STRUCTURE OF A SIGMA70 SUBUNIT FRAGMENT FROM ESCHERICHIA COLI RNA POLYMERASE

PDB ID 1sig

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