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1slt

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(New page: 200px<br /><applet load="1slt" size="450" color="white" frame="true" align="right" spinBox="true" caption="1slt, resolution 1.9&Aring;" /> '''STRUCTURE OF S-LECTIN...)
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[[Image:1slt.jpg|left|200px]]<br /><applet load="1slt" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1slt, resolution 1.9&Aring;" />
 
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'''STRUCTURE OF S-LECTIN, A DEVELOPMENTALLY REGULATED VERTEBRATE BETA-GALACTOSIDE BINDING PROTEIN'''<br />
 
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==Overview==
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==STRUCTURE OF S-LECTIN, A DEVELOPMENTALLY REGULATED VERTEBRATE BETA-GALACTOSIDE BINDING PROTEIN==
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The crystal structure of a 14-kDa bovine spleen S-lectin complexed with, the disaccharide N-acetyllactosamine at 1.9-A resolution reveals a, surprising structural relationship to legume lectins, despite the lack of, sequence homology. Two monomers associate to form an extended, beta-sandwich, each with the same jelly roll topology typical of legume, lectins but with dramatically trimmed loops and with different dimer, association. Each monomer binds one N-acetyllactosamine molecule in a, topologically and spatially different site than that of legume lectins., The carbohydrate-binding site provides an unprecedented paradigm for, carbohydrate binding, with a unique network of salt bridges. The, specificity for beta-galactose arises from intricate interactions that, constrain the position of the O4 atom.
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<StructureSection load='1slt' size='340' side='right'caption='[[1slt]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1slt]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SLT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SLT FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene>, <scene name='pdbligand=OCS:CYSTEINESULFONIC+ACID'>OCS</scene>, <scene name='pdbligand=PRD_900019:N-acetyl-alpha-lactosamine'>PRD_900019</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1slt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1slt OCA], [https://pdbe.org/1slt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1slt RCSB], [https://www.ebi.ac.uk/pdbsum/1slt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1slt ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/LEG1_BOVIN LEG1_BOVIN] May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sl/1slt_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1slt ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1SLT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with CL and CYO as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1SLT OCA].
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*[[Galectin 3D structures|Galectin 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Structure of S-lectin, a developmentally regulated vertebrate beta-galactoside-binding protein., Liao DI, Kapadia G, Ahmed H, Vasta GR, Herzberg O, Proc Natl Acad Sci U S A. 1994 Feb 15;91(4):1428-32. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8108426 8108426]
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[[Category: Bos taurus]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Herzberg, O.]]
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[[Category: Herzberg O]]
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[[Category: Liao, D.I.]]
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[[Category: Liao D-I]]
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[[Category: CL]]
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[[Category: CYO]]
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[[Category: lectin]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 02:28:15 2007''
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Current revision

STRUCTURE OF S-LECTIN, A DEVELOPMENTALLY REGULATED VERTEBRATE BETA-GALACTOSIDE BINDING PROTEIN

PDB ID 1slt

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