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1sr9

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(New page: 200px<br /><applet load="1sr9" size="450" color="white" frame="true" align="right" spinBox="true" caption="1sr9, resolution 2.0&Aring;" /> '''Crystal Structure of ...)
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[[Image:1sr9.gif|left|200px]]<br /><applet load="1sr9" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1sr9, resolution 2.0&Aring;" />
 
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'''Crystal Structure of LeuA from Mycobacterium tuberculosis'''<br />
 
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==Overview==
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==Crystal Structure of LeuA from Mycobacterium tuberculosis==
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The leucine biosynthetic pathway is essential for the growth of, Mycobacterium tuberculosis and is a potential target for the design of new, anti-tuberculosis drugs. The crystal structure of alpha-isopropylmalate, synthase, which catalyzes the first committed step in this pathway, has, been determined by multiwavelength anomalous dispersion methods and, refined at 2.0-A resolution in complex with its substrate, alpha-ketoisovalerate. The structure reveals a tightly associated, domain-swapped dimer in which each monomer comprises an (alpha/beta)(8), TIM barrel catalytic domain, a helical linker domain, and a regulatory, domain of novel fold. Mutational and crystallographic data indicate the, latter as the site for leucine feedback inhibition of activity. Domain, swapping enables the linker domain of one monomer to sit over the, catalytic domain of the other, inserting residues into the active site, that may be important in catalysis. The alpha-ketoisovalerate substrate, binds to an active site zinc ion, adjacent to a cavity that can, accommodate acetyl-CoA. Sequence and structural similarities point to a, catalytic mechanism similar to that of malate synthase and an evolutionary, relationship with an aldolase that catalyzes the reverse reaction on a, similar substrate.
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<StructureSection load='1sr9' size='340' side='right'caption='[[1sr9]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1sr9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SR9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SR9 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=KIV:3-METHYL-2-OXOBUTANOIC+ACID'>KIV</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sr9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sr9 OCA], [https://pdbe.org/1sr9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sr9 RCSB], [https://www.ebi.ac.uk/pdbsum/1sr9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sr9 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/LEU1_MYCTU LEU1_MYCTU] Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sr/1sr9_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sr9 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1SR9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis] with ZN, CL and KIV as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/2-isopropylmalate_synthase 2-isopropylmalate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.3.13 2.3.3.13] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1SR9 OCA].
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*[[2-isopropylmalate synthase 3D structures|2-isopropylmalate synthase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure of LeuA from Mycobacterium tuberculosis, a key enzyme in leucine biosynthesis., Koon N, Squire CJ, Baker EN, Proc Natl Acad Sci U S A. 2004 Jun 1;101(22):8295-300. Epub 2004 May 24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15159544 15159544]
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[[Category: Large Structures]]
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[[Category: 2-isopropylmalate synthase]]
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[[Category: Mycobacterium tuberculosis]]
[[Category: Mycobacterium tuberculosis]]
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[[Category: Single protein]]
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[[Category: Baker EN]]
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[[Category: Baker, E.N.]]
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[[Category: Koon N]]
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[[Category: Koon, N.]]
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[[Category: Squire CJ]]
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[[Category: Squire, C.J.]]
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[[Category: CL]]
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[[Category: KIV]]
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[[Category: ZN]]
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[[Category: tim barrel]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 02:35:48 2007''
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Current revision

Crystal Structure of LeuA from Mycobacterium tuberculosis

PDB ID 1sr9

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