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1hej

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(New page: 200px<br /> <applet load="1hej" size="450" color="white" frame="true" align="right" spinBox="true" caption="1hej" /> '''C-TERMINAL XYLAN BINDING DOMAIN FROM CELLUL...)
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[[Image:1hej.gif|left|200px]]<br />
 
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<applet load="1hej" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1hej" />
 
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'''C-TERMINAL XYLAN BINDING DOMAIN FROM CELLULOMONAS FIMI XYLANASE 11A'''<br />
 
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==Overview==
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==C-terminal xylan binding domain from Cellulomonas fimi xylanase 11A==
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Glycoside hydrolases often contain multiple copies of noncatalytic, carbohydrate binding modules (CBMs) from the same or different families., Currently, the functional importance of this complex molecular, architecture is unclear. To investigate the role of multiple CBMs in plant, cell wall hydrolases, we have determined the polysaccharide binding, properties of wild type and various derivatives of Cellulomonas fimi, xylanase 11A (Cf Xyn11A). This protein, which binds to both cellulose and, xylan, contains two family 2b CBMs that exhibit 70% sequence identity, one, internal (CBM2b-1), which has previously been shown to bind specifically, to xylan and the other at the C-terminus (CBM2b-2). Biochemical, characterization of CBM2b-2 showed that the module bound to insoluble and, soluble oat ... [[http://ispc.weizmann.ac.il/pmbin/getpm?11327868 (full description)]]
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<StructureSection load='1hej' size='340' side='right'caption='[[1hej]]' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1hej]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Cellulomonas_fimi Cellulomonas fimi]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HEJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HEJ FirstGlance]. <br>
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1HEJ is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Cellulomonas_fimi Cellulomonas fimi]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1HEJ OCA]].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hej FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hej OCA], [https://pdbe.org/1hej PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hej RCSB], [https://www.ebi.ac.uk/pdbsum/1hej PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hej ProSAT]</span></td></tr>
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==Reference==
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</table>
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Evidence for synergy between family 2b carbohydrate binding modules in Cellulomonas fimi xylanase 11A., Bolam DN, Xie H, White P, Simpson PJ, Hancock SM, Williamson MP, Gilbert HJ, Biochemistry. 2001 Feb 27;40(8):2468-77. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11327868 11327868]
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== Function ==
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[https://www.uniprot.org/uniprot/XYND_CELFI XYND_CELFI] Endo-acting xylanase which displays no detectable activity against polysaccharides other than xylan. Hydrolyzes glucosidic bonds with retention of anomeric configuration.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/he/1hej_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hej ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Cellulomonas fimi]]
[[Category: Cellulomonas fimi]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Bolam, D.N.]]
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[[Category: Bolam DN]]
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[[Category: Gilbert, H.J.]]
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[[Category: Gilbert HJ]]
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[[Category: Hancock, S.M.]]
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[[Category: Hancock SM]]
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[[Category: Hefang, X.]]
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[[Category: Hefang X]]
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[[Category: Simpson, P.J.]]
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[[Category: Simpson PJ]]
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[[Category: White, P.]]
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[[Category: White P]]
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[[Category: Williamson, M.P.]]
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[[Category: Williamson MP]]
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[[Category: beta-sheet]]
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[[Category: hydrolase]]
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[[Category: xylan binding domain]]
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[[Category: xylanase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Oct 29 21:04:11 2007''
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Current revision

C-terminal xylan binding domain from Cellulomonas fimi xylanase 11A

PDB ID 1hej

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