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1sru

From Proteopedia

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(New page: 200px<br /><applet load="1sru" size="450" color="white" frame="true" align="right" spinBox="true" caption="1sru, resolution 3.30&Aring;" /> '''Crystal structure of...)
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[[Image:1sru.gif|left|200px]]<br /><applet load="1sru" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1sru, resolution 3.30&Aring;" />
 
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'''Crystal structure of full length E. coli SSB protein'''<br />
 
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==Overview==
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==Crystal structure of full length E. coli SSB protein==
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The crystal structure of full-length homotetrameric single-stranded DNA, (ssDNA)-binding protein from Escherichia coli (SSB) has been determined to, 3.3 A resolution and reveals that the entire C-terminal domain is, disordered even in the presence of ssDNA. To our knowledge, this is the, first experimental evidence that the C-terminal domain of SSB may be, inherently disordered. The N-terminal DNA-binding domain of the protein is, well ordered and is virtually indistinguishable from the previously, determined structure of the chymotryptic fragment of SSB (SSBc) in complex, with ssDNA. The absence of observable interactions with the core protein, and the crystal packing of SSB together suggest that the disordered, C-terminal domains likely extend laterally away from the DNA- binding, domains, which may facilitate interactions with components of the, replication machinery in vivo. The structure also reveals the conservation, of molecular contacts between successive tetramers mediated by the L(45), loops as seen in two other crystal forms of SSBc, suggesting a possible, functional relevance of this interaction.
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<StructureSection load='1sru' size='340' side='right'caption='[[1sru]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1sru]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SRU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SRU FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.3&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sru FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sru OCA], [https://pdbe.org/1sru PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sru RCSB], [https://www.ebi.ac.uk/pdbsum/1sru PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sru ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SSB_ECOLI SSB_ECOLI] This protein is essential for replication of the chromosomes and its single-stranded DNA phages. It is also involved in DNA recombination and repair.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sr/1sru_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sru ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1SRU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1SRU OCA].
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*[[Single-stranded DNA-binding protein 3D structures|Single-stranded DNA-binding protein 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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The C-terminal domain of full-length E. coli SSB is disordered even when bound to DNA., Savvides SN, Raghunathan S, Futterer K, Kozlov AG, Lohman TM, Waksman G, Protein Sci. 2004 Jul;13(7):1942-7. Epub 2004 May 28. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15169953 15169953]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Fuetterer, K.]]
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[[Category: Fuetterer K]]
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[[Category: Kozlov, A.G.]]
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[[Category: Kozlov AG]]
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[[Category: Lohman, T.M.]]
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[[Category: Lohman TM]]
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[[Category: Raghunathan, S.]]
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[[Category: Raghunathan S]]
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[[Category: Savvides, S.N.]]
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[[Category: Savvides SN]]
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[[Category: Waksman, G.]]
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[[Category: Waksman G]]
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[[Category: replication]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 02:36:49 2007''
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Current revision

Crystal structure of full length E. coli SSB protein

PDB ID 1sru

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