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1stx

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(New page: 200px<br /><applet load="1stx" size="450" color="white" frame="true" align="right" spinBox="true" caption="1stx, resolution 2.10&Aring;" /> '''Structure of the K38...)
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[[Image:1stx.gif|left|200px]]<br /><applet load="1stx" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1stx, resolution 2.10&Aring;" />
 
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'''Structure of the K38A mutant of EcoRV bound to cognate DNA and Mn2+'''<br />
 
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==Overview==
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==Structure of the K38A mutant of EcoRV bound to cognate DNA and Mn2+==
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Four crystal structures of EcoRV endonuclease mutants K92A and K38A, provide new insight into the mechanism of DNA bending and the structural, basis for metal-dependent phosphodiester bond cleavage. The removal of a, key active site positive charge in the uncleaved K92A-DNA-M(2+) substrate, complex results in binding of a sodium ion in the position of the amine, nitrogen, suggesting a key role for a positive charge at this position in, stabilizing the sharp DNA bend prior to cleavage. By contrast, two, structures of K38A cocrystallized with DNA and Mn(2+) ions in different, lattice environments reveal cleaved product complexes featuring a common, novel conformation of the scissile phosphate group as compared to all, previous EcoRV structures. In these structures, the released 5'-phosphate, and 3'-OH groups remain in close juxtaposition with each other and with, two Mn(2+) ions that bridge the conserved active site carboxylates. The, scissile phosphates are found midway between their positions in the, prereactive substrate and postreactive product complexes of the wild-type, enzyme. Mn(2+) ions occupy two of the three sites previously described in, the prereactive complexes and are plausibly positioned to generate the, nucleophilic hydroxide ion, to compensate for the incipient additional, negative charge in the transition state, and to ionize a second water for, protonation of the 3'-oxyanion. Reconciliation of these findings with, earlier X-ray and fluorescence studies suggests a novel mechanism in which, a single initially bound metal ion in a third distinct site undergoes a, shift in position together with movement of the scissile phosphate deeper, into the active site cleft. This reconfigures the local environment to, permit binding of the second metal ion followed by movement toward the, pentacovalent transition state. The new mechanism suggested here embodies, key features of previously proposed two- and three-metal catalytic models, and offers a view of the stereochemical pathway that integrates much of, the copious structural and functional data that are available from, exhaustive studies in many laboratories.
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<StructureSection load='1stx' size='340' side='right'caption='[[1stx]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1stx]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1STX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1STX FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1stx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1stx OCA], [https://pdbe.org/1stx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1stx RCSB], [https://www.ebi.ac.uk/pdbsum/1stx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1stx ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/T2E5_ECOLX T2E5_ECOLX] Recognizes the double-stranded sequence GATATC and cleaves after T-3.
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==About this Structure==
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==See Also==
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1STX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with MN as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1STX OCA].
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*[[Endonuclease 3D structures|Endonuclease 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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DNA cleavage by EcoRV endonuclease: two metal ions in three metal ion binding sites., Horton NC, Perona JJ, Biochemistry. 2004 Jun 8;43(22):6841-57. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15170321 15170321]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Type II site-specific deoxyribonuclease]]
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[[Category: Horton NC]]
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[[Category: Horton, N.C.]]
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[[Category: Perona JJ]]
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[[Category: Perona, J.J.]]
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[[Category: MN]]
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[[Category: restriction enzyme; ecorv; dna; x-ray crystallography]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 02:39:48 2007''
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Current revision

Structure of the K38A mutant of EcoRV bound to cognate DNA and Mn2+

PDB ID 1stx

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