1sty

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(New page: 200px<br /><applet load="1sty" size="450" color="white" frame="true" align="right" spinBox="true" caption="1sty, resolution 1.67&Aring;" /> '''THE ALPHA ANEURISM: ...)
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[[Image:1sty.jpg|left|200px]]<br /><applet load="1sty" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1sty, resolution 1.67&Aring;" />
 
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'''THE ALPHA ANEURISM: A STRUCTURAL MOTIF REVEALED IN AN INSERTION MUTANT OF STAPHYLOCOCCAL NUCLEASE'''<br />
 
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==Overview==
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==THE ALPHA ANEURISM: A STRUCTURAL MOTIF REVEALED IN AN INSERTION MUTANT OF STAPHYLOCOCCAL NUCLEASE==
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The x-ray crystal structure of a mutant of staphylococcal nuclease that, contains a single glycine residue inserted in the C-terminal alpha-helix, has been solved to 1.67 A resolution and refined to a crystallographic R, value of 0.170. This inserted glycine residue is accommodated in the, alpha-helix by formation of a previously uncharacterized bulge, which we, term the alpha aneurism. A conformational search of known protein, structures has identified the alpha aneurism in a number of protein, families, including the histocompatibility antigens and hemoglobins.
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<StructureSection load='1sty' size='340' side='right'caption='[[1sty]], [[Resolution|resolution]] 1.67&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1sty]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1STY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1STY FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.67&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=THP:THYMIDINE-3,5-DIPHOSPHATE'>THP</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sty FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sty OCA], [https://pdbe.org/1sty PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sty RCSB], [https://www.ebi.ac.uk/pdbsum/1sty PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sty ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NUC_STAAU NUC_STAAU] Enzyme that catalyzes the hydrolysis of both DNA and RNA at the 5' position of the phosphodiester bond.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/st/1sty_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sty ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1STY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus] with CA and THP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Micrococcal_nuclease Micrococcal nuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.31.1 3.1.31.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1STY OCA].
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*[[Staphylococcal nuclease 3D structures|Staphylococcal nuclease 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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The alpha aneurism: a structural motif revealed in an insertion mutant of staphylococcal nuclease., Keefe LJ, Sondek J, Shortle D, Lattman EE, Proc Natl Acad Sci U S A. 1993 Apr 15;90(8):3275-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8475069 8475069]
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[[Category: Large Structures]]
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[[Category: Micrococcal nuclease]]
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[[Category: Single protein]]
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[[Category: Staphylococcus aureus]]
[[Category: Staphylococcus aureus]]
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[[Category: Keefe, L.J.]]
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[[Category: Keefe LJ]]
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[[Category: Lattman, E.E.]]
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[[Category: Lattman EE]]
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[[Category: CA]]
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[[Category: THP]]
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[[Category: hydrolase (phosphoric diester)]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 02:39:53 2007''
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THE ALPHA ANEURISM: A STRUCTURAL MOTIF REVEALED IN AN INSERTION MUTANT OF STAPHYLOCOCCAL NUCLEASE

PDB ID 1sty

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