This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1svy

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="1svy" size="450" color="white" frame="true" align="right" spinBox="true" caption="1svy, resolution 1.75&Aring;" /> '''SEVERIN DOMAIN 2, 1....)
Current revision (08:36, 1 May 2024) (edit) (undo)
 
(16 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1svy.gif|left|200px]]<br /><applet load="1svy" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1svy, resolution 1.75&Aring;" />
 
-
'''SEVERIN DOMAIN 2, 1.75 ANGSTROM CRYSTAL STRUCTURE'''<br />
 
-
==Overview==
+
==SEVERIN DOMAIN 2, 1.75 ANGSTROM CRYSTAL STRUCTURE==
-
The crystal structure of the F-actin binding domain 2 of severin, the, gelsolin homologue from Dictyostelium discoideum, has been determined by, multiple isomorphous replacement and refined to 1.75 A resolution. The, structure reveals an alpha-helix-beta-sheet sandwich similar to the, domains of gelsolin and villin, and contains two cation-binding sites, as, observed in other domain 1 and domain 2 homologues. Comparison of the, structures of several gelsolin family domains has identified residues that, may mediate F-actin binding in gelsolin domain 2 homologues. To assess the, involvement of these residues in F-actin binding, three mutants of human, gelsolin domain 2 were assayed for F-actin binding activity and, thermodynamic stability. Two of the mutants, RRV168AAA and RLK210AAA, demonstrated a lowered affinity for F-actin, indicating a role for those, residues in filament binding. Using both structural and biochemical data, we have constructed a model of the gelsolin domain 1-domain 2-F-actin, complex. This model highlights a number of interactions that may serve as, positive and negative determinants of filament end- and side-binding.
+
<StructureSection load='1svy' size='340' side='right'caption='[[1svy]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1svy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SVY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SVY FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1svy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1svy OCA], [https://pdbe.org/1svy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1svy RCSB], [https://www.ebi.ac.uk/pdbsum/1svy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1svy ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/SEVE_DICDI SEVE_DICDI] Severin blocks the ends of F-actin and causes the fragmentation and depolymerization of actin filaments in a Ca(2+) dependent manner.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sv/1svy_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1svy ConSurf].
 +
<div style="clear:both"></div>
-
==About this Structure==
+
==See Also==
-
1SVY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum] with CA and NA as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1SVY OCA].
+
*[[Severin|Severin]]
-
 
+
__TOC__
-
==Reference==
+
</StructureSection>
-
Mapping the functional surface of domain 2 in the gelsolin superfamily., Puius YA, Fedorov EV, Eichinger L, Schleicher M, Almo SC, Biochemistry. 2000 May 9;39(18):5322-31. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10820002 10820002]
+
[[Category: Dictyostelium discoideum]]
[[Category: Dictyostelium discoideum]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Almo, S.C.]]
+
[[Category: Almo SC]]
-
[[Category: Eichinger, L.]]
+
[[Category: Eichinger L]]
-
[[Category: Fedorov, E.V.]]
+
[[Category: Fedorov EV]]
-
[[Category: Puius, Y.A.]]
+
[[Category: Puius YA]]
-
[[Category: Schleicher, M.]]
+
[[Category: Schleicher M]]
-
[[Category: Sullivan, M.]]
+
[[Category: Sullivan M]]
-
[[Category: CA]]
+
-
[[Category: NA]]
+
-
[[Category: actin-binding protein]]
+
-
[[Category: calcium]]
+
-
[[Category: calcium-binding]]
+
-
[[Category: cytoskeleton]]
+
-
[[Category: gelsolin]]
+
-
[[Category: pip2]]
+
-
[[Category: severin]]
+
-
[[Category: villin]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 02:46:30 2007''
+

Current revision

SEVERIN DOMAIN 2, 1.75 ANGSTROM CRYSTAL STRUCTURE

PDB ID 1svy

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1svy"
Personal tools