2cns

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{{Seed}}
 
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[[Image:2cns.png|left|200px]]
 
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==RimI - Ribosomal S18 N-alpha-protein acetyltransferase in complex with acetylCoA.==
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The line below this paragraph, containing "STRUCTURE_2cns", creates the "Structure Box" on the page.
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<StructureSection load='2cns' size='340' side='right'caption='[[2cns]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2cns]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium_str._LT2 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CNS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CNS FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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{{STRUCTURE_2cns| PDB=2cns | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cns FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cns OCA], [https://pdbe.org/2cns PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cns RCSB], [https://www.ebi.ac.uk/pdbsum/2cns PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cns ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RIMI_SALTY RIMI_SALTY] Acetylates the N-terminal alanine of ribosomal protein S18.[HAMAP-Rule:MF_02210]<ref>PMID:18596200</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cn/2cns_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2cns ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The three ribosomal proteins L7, S5, and S18 are included in the rare subset of prokaryotic proteins that are known to be N(alpha)-acetylated. The GCN5-related N-acetyltransferase (GNAT) protein RimI, responsible for the N(alpha)-acetylation of the ribosomal protein S18, was cloned from Salmonella typhimurium LT2 (RimI(ST)), overexpressed, and purified to homogeneity. Steady-state kinetic parameters for RimI(ST) were determined for AcCoA and a peptide substrate consisting of the first six amino acids of the target protein S18. The crystal structure of RimI(ST) was determined in complex with CoA, AcCoA, and a CoA-S-acetyl-ARYFRR bisubstrate inhibitor. The structures are consistent with a direct nucleophilic addition-elimination mechanism with Glu103 and Tyr115 acting as the catalytic base and acid, respectively. The RimI(ST)-bisubstrate complex suggests that several residues change conformation upon interacting with the N terminus of S18, including Glu103, the proposed active site base, facilitating proton exchange and catalysis.
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===RIMI - RIBOSOMAL S18 N-ALPHA-PROTEIN ACETYLTRANSFERASE IN COMPLEX WITH ACETYLCOA.===
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Crystal structure of RimI from Salmonella typhimurium LT2, the GNAT responsible for N(alpha)-acetylation of ribosomal protein S18.,Vetting MW, Bareich DC, Yu M, Blanchard JS Protein Sci. 2008 Oct;17(10):1781-90. Epub 2008 Jul 2. PMID:18596200<ref>PMID:18596200</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2cns" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_18596200}}, adds the Publication Abstract to the page
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*[[Ribosomal protein S18|Ribosomal protein S18]]
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(as it appears on PubMed at http://www.pubmed.gov), where 18596200 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_18596200}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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2CNS is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium_lt2 Salmonella typhimurium lt2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CNS OCA].
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[[Category: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]]
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[[Category: Bareich DC]]
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==Reference==
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[[Category: Blanchard JS]]
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Crystal Structure of RimI from Salmonella typhimurium LT2, the GNAT responsible for N{alpha}-acetylation of Ribosomal Protein S18., Vetting M, Bareich D, Yu M, Blanchard JS, Protein Sci. 2008 Jul 2;. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18596200 18596200]
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[[Category: Vetting MW]]
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[[Category: Ribosomal-protein-alanine N-acetyltransferase]]
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[[Category: Yu M]]
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[[Category: Salmonella typhimurium lt2]]
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[[Category: Single protein]]
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[[Category: Bareich, D C.]]
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[[Category: Blanchard, J S.]]
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[[Category: Vetting, M W.]]
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[[Category: Yu, M.]]
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[[Category: Acetyltransferase]]
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[[Category: Acyltransferase]]
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[[Category: Gcn5-n-acetyltransferase]]
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[[Category: Gnat]]
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[[Category: N-alpha acetylation]]
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[[Category: Ribosomal protein]]
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[[Category: Transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Oct 1 22:06:15 2008''
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Current revision

RimI - Ribosomal S18 N-alpha-protein acetyltransferase in complex with acetylCoA.

PDB ID 2cns

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