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| - | {{Seed}} | |
| - | [[Image:3eb8.jpg|left|200px]] | |
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| - | <!--
| + | ==VirA== |
| - | The line below this paragraph, containing "STRUCTURE_3eb8", creates the "Structure Box" on the page.
| + | <StructureSection load='3eb8' size='340' side='right'caption='[[3eb8]], [[Resolution|resolution]] 2.40Å' scene=''> |
| - | You may change the PDB parameter (which sets the PDB file loaded into the applet)
| + | == Structural highlights == |
| - | or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
| + | <table><tr><td colspan='2'>[[3eb8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Shigella_flexneri Shigella flexneri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EB8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3EB8 FirstGlance]. <br> |
| - | or leave the SCENE parameter empty for the default display.
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4Å</td></tr> |
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| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3eb8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eb8 OCA], [https://pdbe.org/3eb8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3eb8 RCSB], [https://www.ebi.ac.uk/pdbsum/3eb8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3eb8 ProSAT]</span></td></tr> |
| - | {{STRUCTURE_3eb8| PDB=3eb8 | SCENE= }}
| + | </table> |
| - | | + | == Function == |
| - | ===VirA=== | + | [https://www.uniprot.org/uniprot/VIRA_SHIFL VIRA_SHIFL] Alpha-tubulin-specific protease that is required for entry into epithelial cells and for subsequent intra- and intercellular spreading. Contributes to bacterial entry into epithelial cells by inducing microtubule (MT) destabilization and the formation of membrane ruffles. The membrane ruffling evoked by VirA results from the activation of host rac1, which is associated with the destruction of MT networks. Creates a tunnel inside the host cell cytoplasm by breaking down the microtubule infrastructure. This facilitates the bacterium's movement through the cytoplasm and also helps other bacteria move faster during the invasion of the eukaryotic cell. Is absolutely required for virulence.<ref>PMID:7494473</ref> <ref>PMID:12065406</ref> <ref>PMID:17095701</ref> |
| - | | + | == References == |
| - | | + | <references/> |
| - | <!--
| + | __TOC__ |
| - | The line below this paragraph, {{ABSTRACT_PUBMED_18763811}}, adds the Publication Abstract to the page
| + | </StructureSection> |
| - | (as it appears on PubMed at http://www.pubmed.gov), where 18763811 is the PubMed ID number.
| + | [[Category: Large Structures]] |
| - | -->
| + | |
| - | {{ABSTRACT_PUBMED_18763811}}
| + | |
| - | | + | |
| - | ==About this Structure== | + | |
| - | 3EB8 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Shigella_flexneri Shigella flexneri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EB8 OCA].
| + | |
| - | | + | |
| - | ==Reference== | + | |
| - | Structural and functional studies indicate that Shigella VirA is not a protease and does not directly destabilize microtubules., Germane KL, Ohi R, Goldberg MB, Spiller BW, Biochemistry. 2008 Sep 30;47(39):10241-3. Epub 2008 Sep 3. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18763811 18763811]
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| | [[Category: Shigella flexneri]] | | [[Category: Shigella flexneri]] |
| - | [[Category: Single protein]]
| + | [[Category: Germane KL]] |
| - | [[Category: Germane, K L.]] | + | [[Category: Spiller BW]] |
| - | [[Category: Spiller, B W.]] | + | |
| - | [[Category: Alpha helix]]
| + | |
| - | [[Category: Beta sheet]]
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| - | [[Category: Hydrolase]]
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| - | [[Category: Plasmid]]
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| - | [[Category: Protease]]
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| - | [[Category: Secreted]]
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| - | [[Category: Thiol protease]]
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| - | [[Category: Virulence]]
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| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Oct 1 22:10:45 2008''
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| Structural highlights
Function
VIRA_SHIFL Alpha-tubulin-specific protease that is required for entry into epithelial cells and for subsequent intra- and intercellular spreading. Contributes to bacterial entry into epithelial cells by inducing microtubule (MT) destabilization and the formation of membrane ruffles. The membrane ruffling evoked by VirA results from the activation of host rac1, which is associated with the destruction of MT networks. Creates a tunnel inside the host cell cytoplasm by breaking down the microtubule infrastructure. This facilitates the bacterium's movement through the cytoplasm and also helps other bacteria move faster during the invasion of the eukaryotic cell. Is absolutely required for virulence.[1] [2] [3]
References
- ↑ Uchiya K, Tobe T, Komatsu K, Suzuki T, Watarai M, Fukuda I, Yoshikawa M, Sasakawa C. Identification of a novel virulence gene, virA, on the large plasmid of Shigella, involved in invasion and intercellular spreading. Mol Microbiol. 1995 Jul;17(2):241-50. PMID:7494473
- ↑ Yoshida S, Katayama E, Kuwae A, Mimuro H, Suzuki T, Sasakawa C. Shigella deliver an effector protein to trigger host microtubule destabilization, which promotes Rac1 activity and efficient bacterial internalization. EMBO J. 2002 Jun 17;21(12):2923-35. PMID:12065406 doi:10.1093/emboj/cdf319
- ↑ Yoshida S, Handa Y, Suzuki T, Ogawa M, Suzuki M, Tamai A, Abe A, Katayama E, Sasakawa C. Microtubule-severing activity of Shigella is pivotal for intercellular spreading. Science. 2006 Nov 10;314(5801):985-9. PMID:17095701 doi:10.1126/science.1133174
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