1syy

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="1syy" size="450" color="white" frame="true" align="right" spinBox="true" caption="1syy, resolution 1.70&Aring;" /> '''Crystal structure of...)
Current revision (08:34, 14 February 2024) (edit) (undo)
 
(15 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1syy.gif|left|200px]]<br /><applet load="1syy" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1syy, resolution 1.70&Aring;" />
 
-
'''Crystal structure of the R2 subunit of ribonucleotide reductase from Chlamydia trachomatis'''<br />
 
-
==Overview==
+
==Crystal structure of the R2 subunit of ribonucleotide reductase from Chlamydia trachomatis==
-
Ribonucleotide reductase (RNR) synthesizes the deoxyribonucleotides for, DNA synthesis. The R2 protein of normal class I ribonucleotide reductases, contains a diiron site that produces a stable tyrosyl free radical, essential for enzymatic activity. Structural and electron paramagnetic, resonance studies of R2 from Chlamydia trachomatis reveal a protein, lacking a tyrosyl radical site. Instead, the protein yields an, iron-coupled radical upon reconstitution. The coordinating structure of, the diiron site is similar to that of diiron oxidases/monoxygenases and, supports a role for this radical in the RNR mechanism. The specific ligand, pattern in the C. trachomatis R2 metal site characterizes a new group of, R2 proteins that so far has been found in eight organisms, three of which, are human pathogens.
+
<StructureSection load='1syy' size='340' side='right'caption='[[1syy]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1syy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlamydia_trachomatis Chlamydia trachomatis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SYY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SYY FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=PB:LEAD+(II)+ION'>PB</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1syy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1syy OCA], [https://pdbe.org/1syy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1syy RCSB], [https://www.ebi.ac.uk/pdbsum/1syy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1syy ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/RIR2_CHLTR RIR2_CHLTR] Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides (By similarity).
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sy/1syy_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1syy ConSurf].
 +
<div style="clear:both"></div>
-
==About this Structure==
+
==See Also==
-
1SYY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Chlamydia_trachomatis Chlamydia trachomatis] with FE and PB as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Ribonucleoside-diphosphate_reductase Ribonucleoside-diphosphate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.17.4.1 1.17.4.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1SYY OCA].
+
*[[Ribonucleotide reductase 3D structures|Ribonucleotide reductase 3D structures]]
-
 
+
__TOC__
-
==Reference==
+
</StructureSection>
-
The radical site in chlamydial ribonucleotide reductase defines a new R2 subclass., Hogbom M, Stenmark P, Voevodskaya N, McClarty G, Graslund A, Nordlund P, Science. 2004 Jul 9;305(5681):245-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15247479 15247479]
+
[[Category: Chlamydia trachomatis]]
[[Category: Chlamydia trachomatis]]
-
[[Category: Ribonucleoside-diphosphate reductase]]
+
[[Category: Large Structures]]
-
[[Category: Single protein]]
+
[[Category: Graslund A]]
-
[[Category: Graslund, A.]]
+
[[Category: Hogbom M]]
-
[[Category: Hogbom, M.]]
+
[[Category: McClarty G]]
-
[[Category: McClarty, G.]]
+
[[Category: Nordlund P]]
-
[[Category: Nordlund, P.]]
+
[[Category: Stenmark P]]
-
[[Category: Stenmark, P.]]
+
[[Category: Voevodskaya N]]
-
[[Category: Voevodskaya, N.]]
+
-
[[Category: FE]]
+
-
[[Category: PB]]
+
-
[[Category: diiron; oxygen activation; iron coupled radical; immune evasion]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 02:51:38 2007''
+

Current revision

Crystal structure of the R2 subunit of ribonucleotide reductase from Chlamydia trachomatis

PDB ID 1syy

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1syy"
Personal tools