This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1szu

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="1szu" size="450" color="white" frame="true" align="right" spinBox="true" caption="1szu, resolution 2.52&Aring;" /> '''The structure of gam...)
Current revision (08:36, 1 May 2024) (edit) (undo)
 
(17 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1szu.gif|left|200px]]<br /><applet load="1szu" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1szu, resolution 2.52&Aring;" />
 
-
'''The structure of gamma-aminobutyrate aminotransferase mutant: V241A'''<br />
 
-
==Overview==
+
==The structure of gamma-aminobutyrate aminotransferase mutant: V241A==
-
The E. coli isozyme of gamma-aminobutyrate aminotransferase (GABA-AT) is a, tetrameric pyridoxal phosphate-dependent enzyme that catalyzes, transamination between primary amines and alpha-keto acids. The roles of, the active site residues V241, E211, and I50 in the GABA-AT mechanism have, been probed by site-directed mutagenesis. The beta-branched side chain of, V241 facilitates formation of external aldimine intermediates with primary, amine substrates, while E211 provides charge compensation of R398, selectively in the primary amine half-reaction and I50 forms a hydrophobic, lid at the top of the substrate binding site. The structures of the I50Q, V241A, and E211S mutants were solved by X-ray crystallography to, resolutions of 2.1, 2.5, and 2.52 A, respectively. The structure of, GABA-AT is similar in overall fold and active site structure to that of, dialkylglycine decarboxylase, which catalyzes both transamination and, decarboxylation half-reactions in its normal catalytic cycle. Therefore, an attempt was made to convert GABA-AT into a decarboxylation-dependent, aminotransferase similar to dialkylglycine decarboxylase by systematic, mutation of E. coli GABA-AT active site residues. Two of the twelve, mutants presented, E211S/I50G/C77K and E211S/I50H/V80D, have approximately, 10-fold higher decarboxylation activities than the wild-type enzyme, and, the E211S/I50H/V80D has formally changed the reaction specificity to that, of a decarboxylase.
+
<StructureSection load='1szu' size='340' side='right'caption='[[1szu]], [[Resolution|resolution]] 2.52&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1szu]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SZU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SZU FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.52&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene>, <scene name='pdbligand=PMP:4-DEOXY-4-AMINOPYRIDOXAL-5-PHOSPHATE'>PMP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1szu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1szu OCA], [https://pdbe.org/1szu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1szu RCSB], [https://www.ebi.ac.uk/pdbsum/1szu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1szu ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/GABT_ECOLI GABT_ECOLI] Catalyzes the transfer of the amino group from gamma-aminobutyrate (GABA) to alpha-ketoglutarate (KG) to yield succinic semialdehyde (SSA).<ref>PMID:20639325</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sz/1szu_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1szu ConSurf].
 +
<div style="clear:both"></div>
-
==About this Structure==
+
==See Also==
-
1SZU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with SO4, EDO, PLP and PMP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/4-aminobutyrate_transaminase 4-aminobutyrate transaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.6.1.19 2.6.1.19] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1SZU OCA].
+
*[[Aminotransferase 3D structures|Aminotransferase 3D structures]]
-
 
+
== References ==
-
==Reference==
+
<references/>
-
Kinetic and crystallographic analysis of active site mutants of Escherichia coli gamma-aminobutyrate aminotransferase., Liu W, Peterson PE, Langston JA, Jin X, Zhou X, Fisher AJ, Toney MD, Biochemistry. 2005 Mar 1;44(8):2982-92. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15723541 15723541]
+
__TOC__
-
[[Category: 4-aminobutyrate transaminase]]
+
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Fisher, A.J.]]
+
[[Category: Fisher AJ]]
-
[[Category: Jin, X.]]
+
[[Category: Jin X]]
-
[[Category: Langston, J.A.]]
+
[[Category: Langston JA]]
-
[[Category: Liu, W.]]
+
[[Category: Liu W]]
-
[[Category: Peterson, P.E.]]
+
[[Category: Peterson PE]]
-
[[Category: Toney, M.D.]]
+
[[Category: Toney MD]]
-
[[Category: Zhou, X.]]
+
[[Category: Zhou X]]
-
[[Category: EDO]]
+
-
[[Category: PLP]]
+
-
[[Category: PMP]]
+
-
[[Category: SO4]]
+
-
[[Category: gaba-at]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 02:52:55 2007''
+

Current revision

The structure of gamma-aminobutyrate aminotransferase mutant: V241A

PDB ID 1szu

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1szu"
Personal tools