1szw

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(New page: 200px<br /><applet load="1szw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1szw, resolution 2.00&Aring;" /> '''Crystal structure of...)
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[[Image:1szw.gif|left|200px]]<br /><applet load="1szw" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1szw, resolution 2.00&Aring;" />
 
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'''Crystal structure of E. coli tRNA pseudouridine synthase TruD'''<br />
 
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==Overview==
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==Crystal structure of E. coli tRNA pseudouridine synthase TruD==
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Pseudouridine synthases catalyse the isomerisation of uridine to, pseudouridine in structural RNA. The pseudouridine synthase TruD, that, modifies U13 in tRNA, belongs to a recently identified and large family of, pseudouridine synthases present in all kingdoms of life. We report here, the crystal structure of Escherichia coli TruD at 2.0 A resolution. The, structure reveals an overall V-shaped molecule with an RNA-binding cleft, formed between two domains: a catalytic domain and an insertion domain., The catalytic domain has a fold similar to that of the catalytic domains, of previously characterised pseudouridine synthases, whereas the insertion, domain displays a novel fold.
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<StructureSection load='1szw' size='340' side='right'caption='[[1szw]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1szw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SZW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SZW FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1szw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1szw OCA], [https://pdbe.org/1szw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1szw RCSB], [https://www.ebi.ac.uk/pdbsum/1szw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1szw ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TRUD_ECOLI TRUD_ECOLI] Responsible for synthesis of pseudouridine from uracil-13 in transfer RNAs.<ref>PMID:12756329</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sz/1szw_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1szw ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Pseudouridine synthases catalyse the isomerisation of uridine to pseudouridine in structural RNA. The pseudouridine synthase TruD, that modifies U13 in tRNA, belongs to a recently identified and large family of pseudouridine synthases present in all kingdoms of life. We report here the crystal structure of Escherichia coli TruD at 2.0 A resolution. The structure reveals an overall V-shaped molecule with an RNA-binding cleft formed between two domains: a catalytic domain and an insertion domain. The catalytic domain has a fold similar to that of the catalytic domains of previously characterised pseudouridine synthases, whereas the insertion domain displays a novel fold.
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==About this Structure==
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X-ray structure of tRNA pseudouridine synthase TruD reveals an inserted domain with a novel fold.,Ericsson UB, Nordlund P, Hallberg BM FEBS Lett. 2004 May 7;565(1-3):59-64. PMID:15135053<ref>PMID:15135053</ref>
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1SZW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Active as [http://en.wikipedia.org/wiki/Pseudouridylate_synthase Pseudouridylate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.70 4.2.1.70] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1SZW OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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X-ray structure of tRNA pseudouridine synthase TruD reveals an inserted domain with a novel fold., Ericsson UB, Nordlund P, Hallberg BM, FEBS Lett. 2004 May 7;565(1-3):59-64. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15135053 15135053]
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</div>
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[[Category: Escherichia coli]]
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<div class="pdbe-citations 1szw" style="background-color:#fffaf0;"></div>
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[[Category: Pseudouridylate synthase]]
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[[Category: Single protein]]
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[[Category: Ericsson, U.B.]]
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[[Category: Hallberg, B.M.]]
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[[Category: Nordlund, P.]]
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[[Category: SPINE, Structural.Proteomics.in.Europe.]]
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[[Category: novel fold]]
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[[Category: pseudouridine synthase]]
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[[Category: rna modification]]
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[[Category: spine]]
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[[Category: structural genomics]]
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[[Category: structural proteomics in europe]]
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[[Category: trud]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 02:53:02 2007''
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==See Also==
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*[[Guide-independent Pseudouridine synthase|Guide-independent Pseudouridine synthase]]
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*[[Pseudouridine synthase 3D structures|Pseudouridine synthase 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Escherichia coli]]
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[[Category: Large Structures]]
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[[Category: Ericsson UB]]
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[[Category: Hallberg BM]]
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[[Category: Nordlund P]]

Current revision

Crystal structure of E. coli tRNA pseudouridine synthase TruD

PDB ID 1szw

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