|
|
| (6 intermediate revisions not shown.) |
| Line 1: |
Line 1: |
| - | [[Image:1t4h.jpg|left|200px]]<br /><applet load="1t4h" size="450" color="white" frame="true" align="right" spinBox="true" | + | #REDIRECT [[6cn9]] This PDB entry is obsolete and replaced by 6cn9 |
| - | caption="1t4h, resolution 1.80Å" />
| + | |
| - | '''Crystal structure of the kinase domain of WNK1, a kinase that causes a hereditary form of hypertension'''<br />
| + | |
| - | | + | |
| - | ==Overview==
| + | |
| - | WNK kinases comprise a small group of unique serine/threonine protein, kinases that have been genetically linked to pseudohypoaldosteronism type, II, an autosomal dominant form of hypertension. Here we present the, structure of the kinase domain of WNK1 at 1.8 A resolution, solved in a, low activity conformation. A lysine residue (Lys-233) is found in the, active site emanating from strand beta2 rather than strand beta3 as in, other protein kinases. The activation loop adopts a unique well-folded, inactive conformation. The conformations of the P+1 specificity pocket, the placement of the conserved active site threonine (Thr-386), and the, exterior placement of helix C, contribute to the low activity state. By, homology modeling, we identified two hydrophobic residues in the, substrate-binding groove that contribute to substrate specificity. The, structure of the WNK1 catalytic domain, with its unique active site, may, help in the design of therapeutic reagents for the treatment of, hypertension.
| + | |
| - | | + | |
| - | ==About this Structure==
| + | |
| - | 1T4H is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Active as [http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1T4H OCA].
| + | |
| - | | + | |
| - | ==Reference==
| + | |
| - | Crystal structure of the kinase domain of WNK1, a kinase that causes a hereditary form of hypertension., Min X, Lee BH, Cobb MH, Goldsmith EJ, Structure. 2004 Jul;12(7):1303-11. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15242606 15242606]
| + | |
| - | [[Category: Non-specific serine/threonine protein kinase]]
| + | |
| - | [[Category: Rattus norvegicus]]
| + | |
| - | [[Category: Single protein]]
| + | |
| - | [[Category: Cobb, M.H.]]
| + | |
| - | [[Category: Goldsmith, E.J.]]
| + | |
| - | [[Category: Lee, B.H.]]
| + | |
| - | [[Category: Min, X.]]
| + | |
| - | [[Category: protein serine/threonine kinase]]
| + | |
| - | [[Category: transferase]]
| + | |
| - | [[Category: wnk1]]
| + | |
| - | | + | |
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 02:59:07 2007''
| + | |
