1t4s

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arginase-L-valine complex

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-[[Image:1t4s.gif|left|200px]]<br /><applet load="1t4s" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1t4s, resolution 2.80&Aring;" />
-'''arginase-L-valine complex'''<br />
-==Overview==
+==arginase-L-valine complex==
-Arginase is a manganese metalloenzyme that catalyzes the hydrolysis of, L-arginine to form L-ornithine and urea. The structure and stability of, the binuclear manganese cluster are critical for catalytic activity as it, activates the catalytic nucleophile, metal-bridging hydroxide ion, and, stabilizes the tetrahedral intermediate and its flanking states. Here, we, report X-ray structures of a series of inhibitors bound to the active site, of arginase, and each inhibitor exploits a different mode of coordination, with the Mn(2+)(2) cluster. Specifically, we have studied the binding of, fluoride ion (F(-); an uncompetitive inhibitor) and L-arginine, L-valine, dinor-N(omega)-hydroxy-L-arginine, descarboxy-nor-N(omega)-hydroxy-L-arginine, and, dehydro-2(S)-amino-6-boronohexanoic acid. Some inhibitors, such as, fluoride ion, dinor-N(omega)-hydroxy-L-arginine, and, dehydro-2(S)-amino-6-boronohexanoic acid, cause the net addition of one, ligand to the Mn(2+)(2) cluster. Other inhibitors, such as, descarboxy-nor-N(omega)-hydroxy-L-arginine, simply displace the, metal-bridging hydroxide ion of the native enzyme and do not cause any net, change in the metal coordination polyhedra. The highest affinity, inhibitors displace the metal-bridging hydroxide ion (and sometimes occupy, a Mn(2+)(A) site found vacant in the native enzyme) and maintain a, conserved array of hydrogen bonds with their alpha-amino and -carboxylate, groups.
+<StructureSection load='1t4s' size='340' side='right'caption='[[1t4s]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1t4s]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T4S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1T4S FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=VAL:VALINE'>VAL</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1t4s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t4s OCA], [https://pdbe.org/1t4s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1t4s RCSB], [https://www.ebi.ac.uk/pdbsum/1t4s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1t4s ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/ARGI1_RAT ARGI1_RAT]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/t4/1t4s_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1t4s ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-T4S is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with MN and VAL as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Arginase Arginase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.3.1 3.5.3.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1T4S OCA].
+*[[Arginase 3D structures|Arginase 3D structures]]
+__TOC__
-==Reference==
+</StructureSection>
-Inhibitor coordination interactions in the binuclear manganese cluster of arginase., Cama E, Pethe S, Boucher JL, Han S, Emig FA, Ash DE, Viola RE, Mansuy D, Christianson DW, Biochemistry. 2004 Jul 20;43(28):8987-99. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15248756 15248756]
+[[Category: Large Structures]]
-[[Category: Arginase]]
 [[Category: Rattus norvegicus]]
-[[Category: Single protein]]
+[[Category: Ash DE]]
-[[Category: Ash, D.E.]]
+[[Category: Boucher J-L]]
-[[Category: Boucher, J.L.]]
+[[Category: Cama E]]
-[[Category: Cama, E.]]
+[[Category: Christianson DW]]
-[[Category: Christianson, D.W.]]
+[[Category: Emig FA]]
-[[Category: Emig, F.A.]]
+[[Category: Mansuy D]]
-[[Category: Mansuy, D.]]
+[[Category: Pethe S]]
-[[Category: Pethe, S.]]
+[[Category: Shoufa H]]
-[[Category: Shoufa, H.]]
+[[Category: Viola RE]]
-[[Category: Viola, R.E.]]
-[[Category: MN]]
-[[Category: VAL]]
-[[Category: arginase]]
-[[Category: l-valine]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 02:59:34 2007''

1t4s

From Proteopedia

Current revision

arginase-L-valine complex

Structural highlights

Function

Evolutionary Conservation

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