1t4z

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(New page: 200px<br /><applet load="1t4z" size="450" color="white" frame="true" align="right" spinBox="true" caption="1t4z" /> '''Solution structure of the N-terminal domain ...)
Current revision (06:35, 1 May 2024) (edit) (undo)
 
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[[Image:1t4z.jpg|left|200px]]<br /><applet load="1t4z" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1t4z" />
 
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'''Solution structure of the N-terminal domain of Synechococcus elongatus SasA (25-structures ensemble)'''<br />
 
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==Overview==
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==Solution structure of the N-terminal domain of Synechococcus elongatus SasA (25-structures ensemble)==
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Circadian oscillators are endogenous biological systems that generate the, approximately 24 hour temporal pattern of biological processes and confer, a reproductive fitness advantage to their hosts. The cyanobacterial clock, is the simplest known and the only clock system for which structural, information for core component proteins, in this case KaiA, KaiB and KaiC, is available. SasA, a clock-associated histidine kinase, is necessary for, robustness of the circadian rhythm of gene expression and implicated in, clock output. The N-terminal domain of SasA (N-SasA) interacts directly, with KaiC and likely functions as the sensory domain controlling the SasA, histidine kinase activity. N-SasA and KaiB share significant sequence, similarity and, thus, it has been proposed that they would be structurally, similar and may even compete for KaiC binding. Here, we report the NMR, structure of N-SasA and show it to be different from that of KaiB. The, structural comparisons provide no clear details to suggest competition of, SasA and KaiB for KaiC binding. N-SasA adopts a canonical thioredoxin fold, but lacks the catalytic cysteine residues. A patch of conserved, solvent-exposed residues is found near the canonical thioredoxin active, site. We suggest that this surface is used by N-SasA for protein-protein, interactions. Our analysis suggests that the structural differences, between N-SasA and KaiB are the result of only a few critical amino acid, substitutions.
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<StructureSection load='1t4z' size='340' side='right'caption='[[1t4z]]' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1t4z]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synechococcus_elongatus_PCC_7942_=_FACHB-805 Synechococcus elongatus PCC 7942 = FACHB-805]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T4Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1T4Z FirstGlance]. <br>
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1T4Z is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Synechococcus_elongatus Synechococcus elongatus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1T4Z OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1t4z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t4z OCA], [https://pdbe.org/1t4z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1t4z RCSB], [https://www.ebi.ac.uk/pdbsum/1t4z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1t4z ProSAT]</span></td></tr>
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==Reference==
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</table>
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Structure of the N-terminal domain of the circadian clock-associated histidine kinase SasA., Vakonakis I, Klewer DA, Williams SB, Golden SS, LiWang AC, J Mol Biol. 2004 Sep 3;342(1):9-17. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15313603 15313603]
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== Function ==
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[[Category: Single protein]]
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[https://www.uniprot.org/uniprot/SASA_SYNE7 SASA_SYNE7] May be involved in signal transduction. Participates in the KaiABC clock protein complex, which constitutes the main circadian regulator in cyanobacteria, via its interaction with KaiC. Required for robustness of the circadian rhythm of gene expression and is involved in clock outputs.<ref>PMID:10786837</ref>
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[[Category: Synechococcus elongatus]]
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== Evolutionary Conservation ==
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[[Category: Klewer, D.A.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: LiWang, A.C.]]
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Check<jmol>
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[[Category: Vakonakis, I.]]
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<jmolCheckbox>
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[[Category: alpha/beta protein]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/t4/1t4z_consurf.spt"</scriptWhenChecked>
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[[Category: thioredoxin fold]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 02:59:49 2007''
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1t4z ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Synechococcus elongatus PCC 7942 = FACHB-805]]
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[[Category: Klewer DA]]
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[[Category: LiWang AC]]
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[[Category: Vakonakis I]]

Current revision

Solution structure of the N-terminal domain of Synechococcus elongatus SasA (25-structures ensemble)

PDB ID 1t4z

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