1t54

From Proteopedia

(Difference between revisions)

Current revision

Antibiotic Activity and Structural Analysis of a Scorpion-derived Antimicrobial peptide IsCT and Its Analogs

Structural highlights

1t54 is a 1 chain structure with sequence from Opisthacanthus madagascariensis. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR, 20 models
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

NDB41_OPIMA Shows weak hemolytic activity and antibacterial activity against both Gram-positive and Gram-negative bacteria probably by forming pores in the cell membrane. IsCT adopts an amphipathic alpha-helical structure.^[1] Shows neither hemolytic, nor antibacterial activities, probably because it cannot adopt amphipathic alpha-helical structure.^[2]

Publication Abstract from PubMed

IsCT is a non-cell-selective antimicrobial peptide isolated from the scorpion Opisthacanthus madagascariensis that has potent cytolytic activity against both mammalian and bacterial cells. To investigate the structure-activity relationships of IsCT and to design novel peptide antibiotics with bacterial cell selectivity, we synthesized several analogs of IsCT and determined their three-dimensional structures in solution by 2D-NMR spectroscopy. IsCT has a linear alpha-helical structure from Gly3 to Phe13, and [K7]-IsCT has a linear alpha-helical structure from Leu2 to Phe13. [K7, P8, K11]-IsCT, which has a bend in its middle region, exhibited the highest antibacterial activity without hemolytic activity, suggesting that its proline-induced bend is an important determinant of this selectivity. Tryptophan fluorescence showed that the high selectivity of [K7, P8, K11]-IsCT toward bacterial cells is closely correlated with its highly selective interaction with negatively charged phospholipids. Its potent activity against antibiotic-resistant bacteria suggests that [K7, P8, K11]-IsCT may serve as a promising lead candidate in the development of new peptide antibiotics.

Antibiotic activity and structural analysis of the scorpion-derived antimicrobial peptide IsCT and its analogs.,Lee K, Shin SY, Kim K, Lim SS, Hahm KS, Kim Y Biochem Biophys Res Commun. 2004 Oct 15;323(2):712-9. PMID:15369808^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Dai L, Yasuda A, Naoki H, Corzo G, Andriantsiferana M, Nakajima T. IsCT, a novel cytotoxic linear peptide from scorpion Opisthacanthus madagascariensis. Biochem Biophys Res Commun. 2001 Aug 31;286(4):820-5. PMID:11520071 doi:10.1006/bbrc.2001.5472
↑ Dai L, Corzo G, Naoki H, Andriantsiferana M, Nakajima T. Purification, structure-function analysis, and molecular characterization of novel linear peptides from scorpion Opisthacanthus madagascariensis. Biochem Biophys Res Commun. 2002 May 24;293(5):1514-22. PMID:12054688 doi:10.1016/S0006-291X(02)00423-0
↑ Lee K, Shin SY, Kim K, Lim SS, Hahm KS, Kim Y. Antibiotic activity and structural analysis of the scorpion-derived antimicrobial peptide IsCT and its analogs. Biochem Biophys Res Commun. 2004 Oct 15;323(2):712-9. PMID:15369808 doi:10.1016/j.bbrc.2004.08.144

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1t54"

@@ Line 1: / Line 1: @@
-[[Image:1t54.gif|left|200px]]<br /><applet load="1t54" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1t54" />
-'''Antibiotic Activity and Structural Analysis of a Scorpion-derived Antimicrobial peptide IsCT and Its Analogs'''<br />
-==Overview==
+==Antibiotic Activity and Structural Analysis of a Scorpion-derived Antimicrobial peptide IsCT and Its Analogs==
-IsCT is a non-cell-selective antimicrobial peptide isolated from the, scorpion Opisthacanthus madagascariensis that has potent cytolytic, activity against both mammalian and bacterial cells. To investigate the, structure-activity relationships of IsCT and to design novel peptide, antibiotics with bacterial cell selectivity, we synthesized several, analogs of IsCT and determined their three-dimensional structures in, solution by 2D-NMR spectroscopy. IsCT has a linear alpha-helical structure, from Gly3 to Phe13, and [K7]-IsCT has a linear alpha-helical structure, from Leu2 to Phe13. [K7, P8, K11]-IsCT, which has a bend in its middle, region, exhibited the highest antibacterial activity without hemolytic, activity, suggesting that its proline-induced bend is an important, determinant of this selectivity. Tryptophan fluorescence showed that the, high selectivity of [K7, P8, K11]-IsCT toward bacterial cells is closely, correlated with its highly selective interaction with negatively charged, phospholipids. Its potent activity against antibiotic-resistant bacteria, suggests that [K7, P8, K11]-IsCT may serve as a promising lead candidate, in the development of new peptide antibiotics.
+<StructureSection load='1t54' size='340' side='right'caption='[[1t54]]' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1t54]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Opisthacanthus_madagascariensis Opisthacanthus madagascariensis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T54 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1T54 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1t54 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t54 OCA], [https://pdbe.org/1t54 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1t54 RCSB], [https://www.ebi.ac.uk/pdbsum/1t54 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1t54 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/NDB41_OPIMA NDB41_OPIMA] Shows weak hemolytic activity and antibacterial activity against both Gram-positive and Gram-negative bacteria probably by forming pores in the cell membrane. IsCT adopts an amphipathic alpha-helical structure.<ref>PMID:11520071</ref>   Shows neither hemolytic, nor antibacterial activities, probably because it cannot adopt amphipathic alpha-helical structure.<ref>PMID:12054688</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+IsCT is a non-cell-selective antimicrobial peptide isolated from the scorpion Opisthacanthus madagascariensis that has potent cytolytic activity against both mammalian and bacterial cells. To investigate the structure-activity relationships of IsCT and to design novel peptide antibiotics with bacterial cell selectivity, we synthesized several analogs of IsCT and determined their three-dimensional structures in solution by 2D-NMR spectroscopy. IsCT has a linear alpha-helical structure from Gly3 to Phe13, and [K7]-IsCT has a linear alpha-helical structure from Leu2 to Phe13. [K7, P8, K11]-IsCT, which has a bend in its middle region, exhibited the highest antibacterial activity without hemolytic activity, suggesting that its proline-induced bend is an important determinant of this selectivity. Tryptophan fluorescence showed that the high selectivity of [K7, P8, K11]-IsCT toward bacterial cells is closely correlated with its highly selective interaction with negatively charged phospholipids. Its potent activity against antibiotic-resistant bacteria suggests that [K7, P8, K11]-IsCT may serve as a promising lead candidate in the development of new peptide antibiotics.
-==About this Structure==
+Antibiotic activity and structural analysis of the scorpion-derived antimicrobial peptide IsCT and its analogs.,Lee K, Shin SY, Kim K, Lim SS, Hahm KS, Kim Y Biochem Biophys Res Commun. 2004 Oct 15;323(2):712-9. PMID:15369808<ref>PMID:15369808</ref>
-T54 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Opisthacanthus_madagascariensis Opisthacanthus madagascariensis] with NH2 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1T54 OCA].
-==Reference==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-Antibiotic activity and structural analysis of the scorpion-derived antimicrobial peptide IsCT and its analogs., Lee K, Shin SY, Kim K, Lim SS, Hahm KS, Kim Y, Biochem Biophys Res Commun. 2004 Oct 15;323(2):712-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15369808 15369808]
+</div>
+<div class="pdbe-citations 1t54" style="background-color:#fffaf0;"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
 [[Category: Opisthacanthus madagascariensis]]
-[[Category: Single protein]]
+[[Category: Hahm KS]]
-[[Category: Hahm, K.S.]]
+[[Category: Kim K]]
-[[Category: Kim, K.]]
+[[Category: Kim Y]]
-[[Category: Kim, Y.]]
+[[Category: Lee K]]
-[[Category: Lee, K.]]
+[[Category: Lim SS]]
-[[Category: Lim, S.S.]]
+[[Category: Shin SY]]
-[[Category: Shin, S.Y.]]
-[[Category: NH2]]
-[[Category: coil-helix]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 03:00:02 2007''

1t54

From Proteopedia

Current revision

Antibiotic Activity and Structural Analysis of a Scorpion-derived Antimicrobial peptide IsCT and Its Analogs

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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