2qfr

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{{Seed}}
 
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[[Image:2qfr.jpg|left|200px]]
 
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==Crystal structure of red kidney bean purple acid phosphatase with bound sulfate==
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The line below this paragraph, containing "STRUCTURE_2qfr", creates the "Structure Box" on the page.
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<StructureSection load='2qfr' size='340' side='right'caption='[[2qfr]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2qfr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Phaseolus_vulgaris Phaseolus vulgaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QFR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2QFR FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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{{STRUCTURE_2qfr| PDB=2qfr | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qfr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qfr OCA], [https://pdbe.org/2qfr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qfr RCSB], [https://www.ebi.ac.uk/pdbsum/2qfr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qfr ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PPAF_PHAVU PPAF_PHAVU]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qf/2qfr_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2qfr ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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BACKGROUND: Purple acid phosphatases belong to the family of binuclear metallohydrolases and are involved in a multitude of biological functions, ranging from bacterial killing and bone metabolism in animals to phosphate uptake in plants. Due to its role in bone resorption purple acid phosphatase has evolved into a promising target for the development of anti-osteoporotic chemotherapeutics. The design of specific and potent inhibitors for this enzyme is aided by detailed knowledge of its reaction mechanism. However, despite considerable effort in the last 10 years various aspects of the basic molecular mechanism of action are still not fully understood. RESULTS: Red kidney bean purple acid phosphatase is a heterovalent enzyme with an Fe(III)Zn(II) center in the active site. Two new structures with bound sulfate (2.4 A) and fluoride (2.2 A) provide insight into the pre-catalytic phase of its reaction cycle and phosphorolysis. The sulfate-bound structure illustrates the significance of an extensive hydrogen bonding network in the second coordination sphere in initial substrate binding and orientation prior to hydrolysis. Importantly, both metal ions are five-coordinate in this structure, with only one nucleophilic mu-hydroxide present in the metal-bridging position. The fluoride-bound structure provides visual support for an activation mechanism for this mu-hydroxide whereby substrate binding induces a shift of this bridging ligand towards the divalent metal ion, thus increasing its nucleophilicity. CONCLUSION: In combination with kinetic, crystallographic and spectroscopic data these structures of red kidney bean purple acid phosphatase facilitate the proposal of a comprehensive eight-step model for the catalytic mechanism of purple acid phosphatases in general.
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===Crystal structure of red kidney bean purple acid phosphatase with bound sulfate===
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Crystal structures of a purple acid phosphatase, representing different steps of this enzyme's catalytic cycle.,Schenk G, Elliott TW, Leung E, Carrington LE, Mitic N, Gahan LR, Guddat LW BMC Struct Biol. 2008 Jan 31;8:6. PMID:18234116<ref>PMID:18234116</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2qfr" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_18234116}}, adds the Publication Abstract to the page
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*[[Acid phosphatase 3D structures|Acid phosphatase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 18234116 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_18234116}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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2QFR is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Phaseolus_vulgaris Phaseolus vulgaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QFR OCA].
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==Reference==
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Crystal structures of a purple acid phosphatase, representing different steps of this enzyme's catalytic cycle., Schenk G, Elliott TW, Leung E, Carrington LE, Mitic N, Gahan LR, Guddat LW, BMC Struct Biol. 2008 Jan 31;8:6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18234116 18234116]
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[[Category: Acid phosphatase]]
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[[Category: Phaseolus vulgaris]]
[[Category: Phaseolus vulgaris]]
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[[Category: Single protein]]
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[[Category: Elliot TW]]
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[[Category: Elliot, T W.]]
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[[Category: Gahan LR]]
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[[Category: Gahan, L R.]]
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[[Category: Guddat LW]]
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[[Category: Guddat, L W.]]
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[[Category: Leung E]]
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[[Category: Leung, E.]]
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[[Category: Schenk G]]
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[[Category: Schenk, G.]]
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[[Category: Binuclear metal centre]]
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[[Category: Hydrolase]]
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[[Category: Substrate analog]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Oct 15 14:09:54 2008''
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Current revision

Crystal structure of red kidney bean purple acid phosphatase with bound sulfate

PDB ID 2qfr

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