1t72

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(New page: 200px<br /><applet load="1t72" size="450" color="white" frame="true" align="right" spinBox="true" caption="1t72, resolution 2.9&Aring;" /> '''Crystal structure of ...)
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[[Image:1t72.gif|left|200px]]<br /><applet load="1t72" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1t72, resolution 2.9&Aring;" />
 
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'''Crystal structure of phosphate transport system protein phoU from Aquifex aeolicus'''<br />
 
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==Overview==
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==Crystal structure of phosphate transport system protein phoU from Aquifex aeolicus==
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The phoU gene of Aquifex aeolicus encodes a protein called PHOU_AQUAE with, sequence similarity to the PhoU protein of Escherichia coli. Despite the, fact that there is a large number of family members (more than 300), attributed to almost all known bacteria and despite PHOU_AQUAE's, association with the regulation of genes for phosphate metabolism, the, nature of its regulatory function is not well understood. Nearly one-half, of these PhoU-like proteins, including both PHOU_AQUAE and the one from E., coli, form a subfamily with an apparent dimer structure of two PhoU, domains on the basis of their amino acid sequence. The crystal structure, of PHOU_AQUAE (a 221-amino-acid protein) reveals two similar coiled-coil, PhoU domains, each forming a three-helix bundle. The structures of, PHOU_AQUAE proteins from both a soluble fraction and refolded inclusion, bodies (at resolutions of 2.8 and 3.2A, respectively) showed no, significant differences. The folds of the PhoU domain and Bag domains (for, a class of cofactors of the eukaryotic chaperone Hsp70 family) are, similar. Accordingly, we propose that gene regulation by PhoU may occur by, association of PHOU_AQUAE with the ATPase domain of the histidine kinase, PhoR, promoting release of its substrate PhoB. Other proteins that share, the PhoU domain fold include the coiled-coil domains of the STAT protein, the ribosome-recycling factor, and structural proteins like spectrin.
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<StructureSection load='1t72' size='340' side='right'caption='[[1t72]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1t72]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T72 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1T72 FirstGlance]. <br>
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1T72 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1T72 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1t72 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t72 OCA], [https://pdbe.org/1t72 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1t72 RCSB], [https://www.ebi.ac.uk/pdbsum/1t72 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1t72 ProSAT], [https://www.topsan.org/Proteins/BSGC/1t72 TOPSAN]</span></td></tr>
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Crystal structure of the "PhoU-like" phosphate uptake regulator from Aquifex aeolicus., Oganesyan V, Oganesyan N, Adams PD, Jancarik J, Yokota HA, Kim R, Kim SH, J Bacteriol. 2005 Jun;187(12):4238-44. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15937186 15937186]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PHOU_AQUAE PHOU_AQUAE] Plays a role in the regulation of phosphate uptake. In this role, it may bind, possibly as a chaperone, to PhoR, PhoB or a PhoR-PhoB complex to promote dephosphorylation of phospho-PhoB, or inhibit formation of the PhoR-PhoB transitory complex (Probable).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/t7/1t72_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1t72 ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Aquifex aeolicus]]
[[Category: Aquifex aeolicus]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Adams, P.D.]]
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[[Category: Adams PD]]
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[[Category: BSGC, Berkeley.Structural.Genomics.Center.]]
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[[Category: Jancarik J]]
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[[Category: Jancarik, J.]]
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[[Category: Kim R]]
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[[Category: Kim, R.]]
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[[Category: Kim S-H]]
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[[Category: Kim, S.H.]]
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[[Category: Oganesyan N]]
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[[Category: Oganesyan, N.]]
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[[Category: Oganesyan V]]
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[[Category: Oganesyan, V.]]
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[[Category: berkeley structural genomics center]]
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[[Category: bsgc structure funded by nih]]
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[[Category: helix bundle]]
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[[Category: protein structure initiative]]
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[[Category: psi]]
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[[Category: structural genomics]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 03:02:07 2007''
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Current revision

Crystal structure of phosphate transport system protein phoU from Aquifex aeolicus

PDB ID 1t72

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