1t8k

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="1t8k" size="450" color="white" frame="true" align="right" spinBox="true" caption="1t8k, resolution 1.10&Aring;" /> '''Crystal Structure of...)
Current revision (08:37, 14 February 2024) (edit) (undo)
 
(15 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1t8k.jpg|left|200px]]<br /><applet load="1t8k" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1t8k, resolution 1.10&Aring;" />
 
-
'''Crystal Structure of apo acyl carrier protein from E. coli'''<br />
 
-
==Overview==
+
==Crystal Structure of apo acyl carrier protein from E. coli==
-
A topic of current interest is engineering surface mutations in order to, improve the success rate of protein crystallization. This report explores, the possibility of using metal-ion-mediated crystal-packing interactions, to facilitate rational design. Escherichia coli apo acyl carrier protein, was chosen as a test case because of its high content of negatively, charged carboxylates suitable for metal binding with moderate affinity., The protein was successfully crystallized in the presence of zinc ions., The crystal structure was determined to 1.1 A resolution with MAD phasing, using anomalous signals from the co-crystallized Zn(2+) ions. The case, study suggested an integrated strategy for crystallization and structure, solution of proteins via engineering surface Asp and Glu mutants, crystallizing them in the presence of metal ions such as Zn(2+) and, solving the structures using anomalous signals.
+
<StructureSection load='1t8k' size='340' side='right'caption='[[1t8k]], [[Resolution|resolution]] 1.10&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1t8k]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T8K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1T8K FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.1&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1t8k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t8k OCA], [https://pdbe.org/1t8k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1t8k RCSB], [https://www.ebi.ac.uk/pdbsum/1t8k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1t8k ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/ACP_ECOLI ACP_ECOLI] Carrier of the growing fatty acid chain in fatty acid biosynthesis.[HAMAP-Rule:MF_01217]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/t8/1t8k_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1t8k ConSurf].
 +
<div style="clear:both"></div>
-
==About this Structure==
+
==See Also==
-
1T8K is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with ZN and IMD as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1T8K OCA].
+
*[[Acyl carrier protein 3D structures|Acyl carrier protein 3D structures]]
-
 
+
__TOC__
-
==Reference==
+
</StructureSection>
-
Structure of apo acyl carrier protein and a proposal to engineer protein crystallization through metal ions., Qiu X, Janson CA, Acta Crystallogr D Biol Crystallogr. 2004 Sep;60(Pt 9):1545-54. Epub 2004, Aug 26. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15333924 15333924]
+
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Janson, C.A.]]
+
[[Category: Janson CA]]
-
[[Category: Qiu, X.]]
+
[[Category: Qiu X]]
-
[[Category: IMD]]
+
-
[[Category: ZN]]
+
-
[[Category: acp]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 03:04:15 2007''
+

Current revision

Crystal Structure of apo acyl carrier protein from E. coli

PDB ID 1t8k

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1t8k"
Personal tools