1tad

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(New page: 200px<br /><applet load="1tad" size="450" color="white" frame="true" align="right" spinBox="true" caption="1tad, resolution 1.7&Aring;" /> '''GTPASE MECHANISM OF G...)
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[[Image:1tad.gif|left|200px]]<br /><applet load="1tad" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1tad, resolution 1.7&Aring;" />
 
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'''GTPASE MECHANISM OF GPROTEINS FROM THE 1.7-ANGSTROM CRYSTAL STRUCTURE OF TRANSDUCIN ALPHA-GDP-ALF4-'''<br />
 
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==Overview==
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==GTPASE MECHANISM OF GPROTEINS FROM THE 1.7-ANGSTROM CRYSTAL STRUCTURE OF TRANSDUCIN ALPHA-GDP-ALF4-==
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Aluminium fluoride (AIF-4) activates members of the heterotrimeric, G-protein (G alpha beta gamma) family by binding to inactive G alpha.GDP, near the site occupied by the gamma-phosphate in G alpha.GTP (ref. 3)., Here we describe the crystal structure of transducin alpha.GDP activated, with aluminium fluoride (Gt alpha.GDP.AIF-4.H2O) at 1.7 A, a resolution, sufficient to establish the coordination geometry of the bound aluminium, fluoride as well as the extensive network of direct and water-mediated, interactions that stabilize it. These observations are derived from three, independent representations in the asymmetric unit, eliminating any chance, of drawing conclusions based on stereochemistry imposed by crystal, packing. Surprisingly, aluminium fluoride activates G alpha.GDP by binding, with a geometry resembling a pentavalent intermediate for GTP hydrolysis., The stabilizing interactions involve not only residues that interact with, the gamma-phosphate in Gt alpha.GTP gamma S, but also conserved residues, for GTPase activity. Thus the Gt alpha.GDP.AIF-4.H2O structure provides, new insight into the mechanism of GTP hydrolysis.
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<StructureSection load='1tad' size='340' side='right'caption='[[1tad]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1tad]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TAD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TAD FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ALF:TETRAFLUOROALUMINATE+ION'>ALF</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CAC:CACODYLATE+ION'>CAC</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tad FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tad OCA], [https://pdbe.org/1tad PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tad RCSB], [https://www.ebi.ac.uk/pdbsum/1tad PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tad ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GNAT1_BOVIN GNAT1_BOVIN] Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Transducin is an amplifier and one of the transducers of a visual impulse that performs the coupling between rhodopsin and cGMP-phosphodiesterase.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ta/1tad_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tad ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1TAD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with CA, CAC, ALF and GDP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1TAD OCA].
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*[[Transducin 3D structures|Transducin 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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GTPase mechanism of Gproteins from the 1.7-A crystal structure of transducin alpha-GDP-AIF-4., Sondek J, Lambright DG, Noel JP, Hamm HE, Sigler PB, Nature. 1994 Nov 17;372(6503):276-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7969474 7969474]
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[[Category: Bos taurus]]
[[Category: Bos taurus]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Hamm, H.E.]]
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[[Category: Hamm HE]]
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[[Category: Lambright, D.G.]]
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[[Category: Lambright DG]]
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[[Category: Noel, J.P.]]
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[[Category: Noel JP]]
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[[Category: Sigler, P.B.]]
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[[Category: Sigler PB]]
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[[Category: Sondek, J.]]
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[[Category: Sondek J]]
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[[Category: ALF]]
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[[Category: CA]]
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[[Category: CAC]]
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[[Category: GDP]]
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[[Category: gtp-binding protein]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 03:06:58 2007''
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Current revision

GTPASE MECHANISM OF GPROTEINS FROM THE 1.7-ANGSTROM CRYSTAL STRUCTURE OF TRANSDUCIN ALPHA-GDP-ALF4-

PDB ID 1tad

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