3eg9

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{{Seed}}
 
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[[Image:3eg9.jpg|left|200px]]
 
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==Crystal structure of the mammalian COPII-coat protein Sec23/24 bound to the transport signal sequence of membrin==
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The line below this paragraph, containing "STRUCTURE_3eg9", creates the "Structure Box" on the page.
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<StructureSection load='3eg9' size='340' side='right'caption='[[3eg9]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3eg9]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EG9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3EG9 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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{{STRUCTURE_3eg9| PDB=3eg9 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3eg9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eg9 OCA], [https://pdbe.org/3eg9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3eg9 RCSB], [https://www.ebi.ac.uk/pdbsum/3eg9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3eg9 ProSAT]</span></td></tr>
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</table>
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== Disease ==
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[https://www.uniprot.org/uniprot/SC23A_HUMAN SC23A_HUMAN] Defects in SEC23A are the cause of craniolenticulosutural dysplasia (CLSD) [MIM:[https://omim.org/entry/607812 607812]; also known as cranio-lenticulo-sutural dysplasia. CLSD is an autosomal recessive syndrome characterized by late-closing fontanels, sutural cataracts, facial dysmorphisms and skeletal defects.<ref>PMID:16980979</ref>
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== Function ==
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[https://www.uniprot.org/uniprot/SC23A_HUMAN SC23A_HUMAN] Component of the COPII coat, that covers ER-derived vesicles involved in transport from the endoplasmic reticulum to the Golgi apparatus. COPII acts in the cytoplasm to promote the transport of secretory, plasma membrane, and vacuolar proteins from the endoplasmic reticulum to the Golgi complex.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eg/3eg9_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3eg9 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Genomic analysis shows that the increased complexity of trafficking pathways in mammalian cells involves an expansion of the number of SNARE, Rab and COP proteins. Thus, the human genome encodes four forms of Sec24, the cargo selection subunit of the COPII vesicular coat, and this is proposed to increase the range of cargo accommodated by human COPII-coated vesicles. In this study, we combined X-ray crystallographic and biochemical analysis with functional assays of cargo packaging into COPII vesicles to establish molecular mechanisms for cargo discrimination by human Sec24 subunits. A conserved IxM packaging signal binds in a surface groove of Sec24c and Sec24d, but the groove is occluded in the Sec24a and Sec24b subunits. Conversely, LxxLE class transport signals and the DxE signal of VSV glycoprotein are selectively bound by Sec24a and Sec24b subunits. A comparative analysis of crystal structures of the four human Sec24 isoforms establishes the structural determinants for discrimination among these transport signals, and provides a framework to understand how an expansion of coat subunits extends the range of cargo proteins packaged into COPII-coated vesicles.
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===Crystal structure of the mammalian COPII-coat protein Sec23/24 bound to the transport signal sequence of membrin===
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Structural basis of cargo membrane protein discrimination by the human COPII coat machinery.,Mancias JD, Goldberg J EMBO J. 2008 Nov 5;27(21):2918-28. Epub 2008 Oct 9. PMID:18843296<ref>PMID:18843296</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The line below this paragraph, {{ABSTRACT_PUBMED_18843296}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 3eg9" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 18843296 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_18843296}}
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__TOC__
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</StructureSection>
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==Disease==
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Known disease associated with this structure: Craniolenticulosutural dysplasia OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=610511 610511]]
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==About this Structure==
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3EG9 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EG9 OCA].
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==Reference==
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Structural basis of cargo membrane protein discrimination by the human COPII coat machinery., Mancias JD, Goldberg J, EMBO J. 2008 Oct 9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18843296 18843296]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Goldberg, J.]]
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[[Category: Goldberg J]]
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[[Category: Mancias, J D.]]
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[[Category: Mancias JD]]
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[[Category: Copii coat]]
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[[Category: Disease mutation]]
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[[Category: Endoplasmic reticulum]]
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[[Category: Er-golgi transport]]
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[[Category: Golgi apparatus]]
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[[Category: Membrane]]
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[[Category: Polymorphism]]
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[[Category: Protein transport]]
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[[Category: Transport]]
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[[Category: Transport signal sequence]]
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[[Category: Vesicle transport]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Oct 22 10:55:11 2008''
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Current revision

Crystal structure of the mammalian COPII-coat protein Sec23/24 bound to the transport signal sequence of membrin

PDB ID 3eg9

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