This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1tcb

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="1tcb" size="450" color="white" frame="true" align="right" spinBox="true" caption="1tcb, resolution 2.1&Aring;" /> '''THE SEQUENCE, CRYSTAL...)
Current revision (08:39, 1 May 2024) (edit) (undo)
 
(18 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1tcb.gif|left|200px]]<br /><applet load="1tcb" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1tcb, resolution 2.1&Aring;" />
 
-
'''THE SEQUENCE, CRYSTAL STRUCTURE DETERMINATION AND REFINEMENT OF TWO CRYSTAL FORMS OF LIPASE B FROM CANDIDA ANTARCTICA'''<br />
 
-
==Overview==
+
==THE SEQUENCE, CRYSTAL STRUCTURE DETERMINATION AND REFINEMENT OF TWO CRYSTAL FORMS OF LIPASE B FROM CANDIDA ANTARCTICA==
-
BACKGROUND: Lipases constitute a family of enzymes that hydrolyze, triglycerides. They occur in many organisms and display a wide variety of, substrate specificities. In recent years, much progress has been made, towards explaining the mechanism of these enzymes and their ability to, hydrolyze their substrates at an oil-water interface. RESULTS: We have, determined the DNA and amino acid sequences for lipase B from the yeast, Candida antarctica. The primary sequence has no significant homology to, any other known lipase and deviates from the consensus sequence around the, active site serine that is found in other lipases. We have determined the, crystal structure of this enzyme using multiple isomorphous replacement, methods for two crystal forms. Models for the orthorhombic and monoclinic, crystal forms of the enzyme have been refined to 1.55 A and 2.1 A, resolution, respectively. Lipase B is an alpha/beta type protein that has, many features in common with previously determined lipase structures and, other related enzymes. In the monoclinic crystal form, lipid-like, molecules, most likely beta-octyl glucoside, can be seen close to the, active site. The behaviour of these lipid molecules in the crystal, structure has been studied at different pH values. CONCLUSION: The, structure of Candida antarctica lipase B shows that the enzyme has a, Ser-His-Asp catalytic triad in its active site. The structure appears to, be in an 'open' conformation with a rather restricted entrance to the, active site. We believe that this accounts for the substrate specificity, and high degree of stereospecificity of this lipase.
+
<StructureSection load='1tcb' size='340' side='right'caption='[[1tcb]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1tcb]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Moesziomyces_antarcticus Moesziomyces antarcticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TCB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TCB FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tcb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tcb OCA], [https://pdbe.org/1tcb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tcb RCSB], [https://www.ebi.ac.uk/pdbsum/1tcb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tcb ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/LIPB_PSEA2 LIPB_PSEA2] Hydrolysis of triglycerides. Is very stereospecific both in hydrolysis and in organic synthesis and has a potentially important application in glucolipid synthesis.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tc/1tcb_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tcb ConSurf].
 +
<div style="clear:both"></div>
-
==About this Structure==
+
==See Also==
-
1TCB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with BOG as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1TCB OCA].
+
*[[Lipase 3D Structures|Lipase 3D Structures]]
-
 
+
__TOC__
-
==Reference==
+
</StructureSection>
-
The sequence, crystal structure determination and refinement of two crystal forms of lipase B from Candida antarctica., Uppenberg J, Hansen MT, Patkar S, Jones TA, Structure. 1994 Apr 15;2(4):293-308. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8087556 8087556]
+
[[Category: Large Structures]]
-
[[Category: Single protein]]
+
[[Category: Moesziomyces antarcticus]]
-
[[Category: Triacylglycerol lipase]]
+
[[Category: Jones TA]]
-
[[Category: Jones, T.A.]]
+
[[Category: Uppenberg J]]
-
[[Category: Uppenberg, J.]]
+
-
[[Category: BOG]]
+
-
[[Category: hydrolase(carboxylic esterase)]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 03:09:00 2007''
+

Current revision

THE SEQUENCE, CRYSTAL STRUCTURE DETERMINATION AND REFINEMENT OF TWO CRYSTAL FORMS OF LIPASE B FROM CANDIDA ANTARCTICA

PDB ID 1tcb

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1tcb"
Personal tools