1tfr

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="1tfr" size="450" color="white" frame="true" align="right" spinBox="true" caption="1tfr, resolution 2.06&Aring;" /> '''RNASE H FROM BACTERI...)
Current revision (08:39, 14 February 2024) (edit) (undo)
 
(16 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1tfr.gif|left|200px]]<br /><applet load="1tfr" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1tfr, resolution 2.06&Aring;" />
 
-
'''RNASE H FROM BACTERIOPHAGE T4'''<br />
 
-
==Overview==
+
==RNASE H FROM BACTERIOPHAGE T4==
-
Bacteriophage T4 RNase H is a 5' to 3' exonuclease that removes RNA, primers from the lagging strand of the DNA replication fork and is a, member of the RAD2 family of eukaryotic and prokaryotic replication and, repair nucleases. The crystal structure of the full-length native form of, T4 RNase H has been solved at 2.06 angstroms resolution in the presence of, Mg2+ but in the absence of nucleic acids. The most conserved residues are, clustered together in a large cleft with two Mg2+ in the proposed active, site. This structure suggests the way in which the widely separated, conserved regions in the larger nucleotide excision repair proteins, such, as human XPG, could assemble into a structure like that of the smaller, replication nucleases.
+
<StructureSection load='1tfr' size='340' side='right'caption='[[1tfr]], [[Resolution|resolution]] 2.06&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1tfr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_T4 Escherichia virus T4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TFR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TFR FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.06&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tfr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tfr OCA], [https://pdbe.org/1tfr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tfr RCSB], [https://www.ebi.ac.uk/pdbsum/1tfr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tfr ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/RNH_BPT4 RNH_BPT4] 5' to 3' exonuclease that removes the pentamer RNA primers from DNA chains initiated by the T4 primase-helicase.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tf/1tfr_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tfr ConSurf].
 +
<div style="clear:both"></div>
-
==About this Structure==
+
==See Also==
-
1TFR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t4 Bacteriophage t4] with MG as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Ribonuclease_H Ribonuclease H], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.26.4 3.1.26.4] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1TFR OCA].
+
*[[Ribonuclease 3D structures|Ribonuclease 3D structures]]
-
 
+
__TOC__
-
==Reference==
+
</StructureSection>
-
Structure of bacteriophage T4 RNase H, a 5' to 3' RNA-DNA and DNA-DNA exonuclease with sequence similarity to the RAD2 family of eukaryotic proteins., Mueser TC, Nossal NG, Hyde CC, Cell. 1996 Jun 28;85(7):1101-12. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8674116 8674116]
+
[[Category: Escherichia virus T4]]
-
[[Category: Bacteriophage t4]]
+
[[Category: Large Structures]]
-
[[Category: Ribonuclease H]]
+
[[Category: Hyde CC]]
-
[[Category: Single protein]]
+
[[Category: Mueser TC]]
-
[[Category: Hyde, C.C.]]
+
[[Category: Nossal NG]]
-
[[Category: Mueser, T.C.]]
+
-
[[Category: Nossal, N.G.]]
+
-
[[Category: MG]]
+
-
[[Category: 5u-3u exonuclease]]
+
-
[[Category: dna:dna]]
+
-
[[Category: hydrolase (nucleic acid)]]
+
-
[[Category: magnesium-containing]]
+
-
[[Category: metal-dependent]]
+
-
[[Category: rna:rna]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 03:13:46 2007''
+

Current revision

RNASE H FROM BACTERIOPHAGE T4

PDB ID 1tfr

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1tfr"
Personal tools