1tfu

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(New page: 200px<br /><applet load="1tfu" size="450" color="white" frame="true" align="right" spinBox="true" caption="1tfu, resolution 1.99&Aring;" /> '''phosphopantetheine a...)
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[[Image:1tfu.gif|left|200px]]<br /><applet load="1tfu" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1tfu, resolution 1.99&Aring;" />
 
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'''phosphopantetheine adenylyltransferase from Mycobacterium tuberculosis'''<br />
 
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==Overview==
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==phosphopantetheine adenylyltransferase from Mycobacterium tuberculosis==
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Phosphopantetheine adenylyltransferase (PPAT) catalyzes the penultimate, step in prokaryotic coenzyme A (CoA) biosynthesis, directing the transfer, of an adenylyl group from ATP to 4'-phosphopantetheine (Ppant) to yield, dephospho-CoA (dPCoA). The crystal structures of Escherichia coli PPAT, bound to its substrates, product, and inhibitor revealed an allosteric, hexameric enzyme with half-of-sites reactivity, and established an in-line, displacement catalytic mechanism. To provide insight into the mechanism of, ligand binding we solved the apoenzyme (Apo) crystal structure of PPAT, from Mycobacterium tuberculosis. In its Apo form, PPAT is a symmetric, hexamer with an open solvent channel. However, ligand binding provokes, asymmetry and alters the structure of the solvent channel, so that ligand, binding becomes restricted to one trimer.
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<StructureSection load='1tfu' size='340' side='right'caption='[[1tfu]], [[Resolution|resolution]] 1.99&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1tfu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TFU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TFU FirstGlance]. <br>
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1TFU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Active as [http://en.wikipedia.org/wiki/Pantetheine-phosphate_adenylyltransferase Pantetheine-phosphate adenylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.3 2.7.7.3] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1TFU OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.99&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tfu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tfu OCA], [https://pdbe.org/1tfu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tfu RCSB], [https://www.ebi.ac.uk/pdbsum/1tfu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tfu ProSAT]</span></td></tr>
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==Reference==
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</table>
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Substrate-induced asymmetry and channel closure revealed by the apoenzyme structure of Mycobacterium tuberculosis phosphopantetheine adenylyltransferase., Morris VK, Izard T, Protein Sci. 2004 Sep;13(9):2547-52. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15322293 15322293]
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== Function ==
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[https://www.uniprot.org/uniprot/COAD_MYCTU COAD_MYCTU] Reversibly transfers an adenylyl group from ATP to 4'-phosphopantetheine, yielding dephospho-CoA (dPCoA) and pyrophosphate (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tf/1tfu_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tfu ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Mycobacterium tuberculosis]]
[[Category: Mycobacterium tuberculosis]]
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[[Category: Pantetheine-phosphate adenylyltransferase]]
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[[Category: Izard T]]
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[[Category: Single protein]]
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[[Category: Morris VK]]
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[[Category: Izard, T.]]
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[[Category: Morris, V.K.]]
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[[Category: TBSGC, TB.Structural.Genomics.Consortium.]]
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[[Category: protein structure initiative]]
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[[Category: psi]]
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[[Category: structural genomics]]
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[[Category: tb structural genomics consortium]]
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[[Category: tbsgc]]
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[[Category: transport protein]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 03:13:53 2007''
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Current revision

phosphopantetheine adenylyltransferase from Mycobacterium tuberculosis

PDB ID 1tfu

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