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1thq

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(New page: 200px<br /><applet load="1thq" size="450" color="white" frame="true" align="right" spinBox="true" caption="1thq, resolution 1.90&Aring;" /> '''Crystal Structure of...)
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[[Image:1thq.jpg|left|200px]]<br /><applet load="1thq" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1thq, resolution 1.90&Aring;" />
 
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'''Crystal Structure of Outer Membrane Enzyme PagP'''<br />
 
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==Overview==
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==Crystal Structure of Outer Membrane Enzyme PagP==
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The ability of enzymes to distinguish between fatty acyl groups can, involve molecular measuring devices termed hydrocarbon rulers, but the, molecular basis for acyl-chain recognition in any membrane-bound enzyme, remains to be defined. PagP is an outer membrane acyltransferase that, helps pathogenic bacteria to evade the host immune response by, transferring a palmitate chain from a phospholipid to lipid A (endotoxin)., PagP can distinguish lipid acyl chains that differ by a single methylene, unit, indicating that the enzyme possesses a remarkably precise, hydrocarbon ruler. We present the 1.9 A crystal structure of PagP, an, eight-stranded beta-barrel with an unexpected interior hydrophobic pocket, that is occupied by a single detergent molecule. The buried detergent is, oriented normal to the presumed plane of the membrane, whereas the PagP, beta-barrel axis is tilted by approximately 25 degrees. Acyl group, specificity is modulated by mutation of Gly88 lining the bottom of the, hydrophobic pocket, thus confirming the hydrocarbon ruler mechanism for, palmitate recognition. A striking structural similarity between PagP and, the lipocalins suggests an evolutionary link between these proteins.
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<StructureSection load='1thq' size='340' side='right'caption='[[1thq]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1thq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1THQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1THQ FirstGlance]. <br>
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1THQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with ACT, LDA and MPD as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1THQ OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=LDA:LAURYL+DIMETHYLAMINE-N-OXIDE'>LDA</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1thq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1thq OCA], [https://pdbe.org/1thq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1thq RCSB], [https://www.ebi.ac.uk/pdbsum/1thq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1thq ProSAT]</span></td></tr>
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A hydrocarbon ruler measures palmitate in the enzymatic acylation of endotoxin., Ahn VE, Lo EI, Engel CK, Chen L, Hwang PM, Kay LE, Bishop RE, Prive GG, EMBO J. 2004 Aug 4;23(15):2931-41. Epub 2004 Jul 22. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15272304 15272304]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PAGP_ECOLI PAGP_ECOLI] PagP is required both for biosynthesis of hepta-acylated lipid A species containing palmitate and for resistance to cationic antimicrobial peptides (CAMPs). It catalyzes the transfer of a palmitate chain (16:0) from the sn-1 position of a glycerophospholipid to the free hydroxyl group of the (R)-3-hydroxymyristate chain at position 2 of lipid A (endotoxin). Modifications of lipid A with a palmitate chain allow to evade host immune defenses by resisting antimicrobial peptides and attenuating the inflammatory response to infection triggered by lipopolysaccharide through the Toll-like receptor 4 (TLR4) signal transduction pathway. Phosphatidylglycerol (PtdGro), phosphatidylethanolamine (PtdEtn), phosphatidylserine (PtdSer) and phosphatidic acid (Ptd-OH) are all effective acyl donors.<ref>PMID:11013210</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/th/1thq_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1thq ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Ahn, V.E.]]
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[[Category: Ahn VE]]
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[[Category: Bishop, R.E.]]
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[[Category: Bishop RE]]
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[[Category: Chen, L.]]
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[[Category: Chen L]]
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[[Category: Engel, C.K.]]
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[[Category: Engel CK]]
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[[Category: Hwang, P.M.]]
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[[Category: Hwang PM]]
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[[Category: Kay, L.E.]]
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[[Category: Kay LE]]
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[[Category: Lo, E.I.]]
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[[Category: Lo EI]]
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[[Category: Prive, G.G.]]
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[[Category: Prive GG]]
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[[Category: ACT]]
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[[Category: LDA]]
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[[Category: MPD]]
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[[Category: beta-barrel]]
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[[Category: outer membrane enzyme]]
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[[Category: pagp]]
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[[Category: palmitoyltransferase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 03:16:28 2007''
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Current revision

Crystal Structure of Outer Membrane Enzyme PagP

PDB ID 1thq

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